9W0O

cryoEM structure of S1P3 in complex with heterotrimeric G protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38745.4	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	54	6000.0	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D (D)	Sphingosine 1-phosphate receptor 3	polymer	430	47985.6	1	UniProt (Q99500)	Homo sapiens (human)	S1P receptor 3,S1P3,Endothelial differentiation G-protein coupled receptor 3,Sphingosine 1-phosphate receptor Edg-3,S1P receptor Edg-3

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Glu 6	Gln 6	engineered mutation
Gln 130	Glu 130	engineered mutation
Asp 237	Asn 237	engineered mutation

C: 9 - 62 (UniProt: P59768)

PDB	External Database	Details
Gln 17	Glu 17	engineered mutation
Gln 58	Glu 58	engineered mutation

D: 27 - 404 (UniProt: Q99500)

PDB	External Database	Details
Met 11	-	initiating methionine
Lys 12	-	expression tag
Thr 13	-	expression tag
Ile 14	-	expression tag
Ile 15	-	expression tag
Ala 16	-	expression tag
Leu 17	-	expression tag
Ser 18	-	expression tag
Tyr 19	-	expression tag
Ile 20	-	expression tag
Phe 21	-	expression tag
Cys 22	-	expression tag
Leu 23	-	expression tag
Val 24	-	expression tag
Phe 25	-	expression tag
Ala 26	-	expression tag
Trp 405	-	expression tag
Ser 406	-	expression tag
His 407	-	expression tag
Pro 408	-	expression tag
Gln 409	-	expression tag
Phe 410	-	expression tag
Glu 411	-	expression tag
Lys 412	-	expression tag
Gly 413	-	expression tag
Gly 414	-	expression tag
Gly 415	-	expression tag
Ser 416	-	expression tag
Gly 417	-	expression tag
Gly 418	-	expression tag
Gly 419	-	expression tag
Ser 420	-	expression tag
Gly 421	-	expression tag
Gly 422	-	expression tag
Ser 423	-	expression tag
Ala 424	-	expression tag
Trp 425	-	expression tag
Ser 426	-	expression tag
His 427	-	expression tag
Pro 428	-	expression tag
Gln 429	-	expression tag
Phe 430	-	expression tag
Glu 431	-	expression tag
Lys 432	-	expression tag
Asp 433	-	expression tag
Tyr 434	-	expression tag
Lys 435	-	expression tag
Asp 436	-	expression tag
Asp 437	-	expression tag
Asp 438	-	expression tag
Asp 439	-	expression tag
Lys 440	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	133178.1
All*	Total formula weight	133178.1

*Water molecules are not included.