9W05

Crystal structure of C-terminal domain of theta type Carbonic Anhydrase 3 (A88D mutant) from marine diatom Phaeodactylum tricornutum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Limiting CO2-inducible protein B/C beta carbonyic anhydrase domain-containing protein	polymer	264	28094.7	2	UniProt (A0A173M023)	Phaeodactylum tricornutum
2	C (A)	CARBON DIOXIDE	non-polymer		44.0	1	Chemie (CO2) PubChem (280)
3	D, E, H, I (A, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA) PubChem (923)
4	F, J (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)
5	G (B)	BICARBONATE ION	non-polymer		61.0	1	Chemie (BCT) PubChem (769)
6	K, L (A, B)	water	water		18.0	238	Chemie (HOH)

Sequence modifications

A, B: 2 - 249 (UniProt: A0A173M023)

PDB	External Database	Details
Met -14	-	initiating methionine
Asn -13	-	expression tag
His -12	-	expression tag
Lys -11	-	expression tag
Val -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ile -3	-	expression tag
Glu -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Ser 1	-	expression tag
Asp 88	Ala 384	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56189.4
Non-Polymers*	Number of molecules	8
	Total formula weight	327.8
All*	Total formula weight	56517.2

*Water molecules are not included.