9W02

Cryo-EM structure of TasH-tigRNA-ssDNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	HNH nuclease domain-containing protein	polymer	354	40031.2	2	UniProt (M4R212)	Salicola phage CGphi29
2	C (C)	RNA (36-MER)	polymer	37	11948.2	1		Salicola phage CGphi29
3	D (D)	DNA (38-MER)	polymer	38	11784.6	1		Salicola phage CGphi29

Sequence modifications

A, B: 1 - 309 (UniProt: M4R212)

PDB	External Database	Details
Gly 310	-	expression tag
Gly 311	-	expression tag
Ser 312	-	expression tag
Trp 313	-	expression tag
Ser 314	-	expression tag
His 315	-	expression tag
Pro 316	-	expression tag
Gln 317	-	expression tag
Phe 318	-	expression tag
Glu 319	-	expression tag
Lys 320	-	expression tag
Gly 321	-	expression tag
Gly 322	-	expression tag
Gly 323	-	expression tag
Ser 324	-	expression tag
Gly 325	-	expression tag
Gly 326	-	expression tag
Gly 327	-	expression tag
Ser 328	-	expression tag
Gly 329	-	expression tag
Gly 330	-	expression tag
Ser 331	-	expression tag
Ala 332	-	expression tag
Trp 333	-	expression tag
Ser 334	-	expression tag
His 335	-	expression tag
Pro 336	-	expression tag
Gln 337	-	expression tag
Phe 338	-	expression tag
Glu 339	-	expression tag
Lys 340	-	expression tag
Asn 341	-	expression tag
Leu 342	-	expression tag
Tyr 343	-	expression tag
Phe 344	-	expression tag
Gln 345	-	expression tag
Ser 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	103795.3
All*	Total formula weight	103795.3

*Water molecules are not included.