9VUM

Cryo-EM structure of the Nipah virus RNA-dependent RNA polymerase complex bound to allosteric inhibitor ERDRP-0519

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (L)	Maltose/maltodextrin-binding periplasmic protein,RNA-directed RNA polymerase L	polymer	1904	215827.8	1	UniProt (P0AEX9) UniProt (Q997F0) Pfam (PF01547)	Escherichia coli K-12	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Protein L,Large structural protein,Replicase,Transcriptase
2	B, C, D, E (A, B, C, D)	Maltose/maltodextrin-binding periplasmic protein,Phosphoprotein	polymer	638	70995.8	4	UniProt (P0AEX9) UniProt (Q9IK91) Pfam (PF01547)	Escherichia coli K-12	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Protein P
3	F, G (L)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)
4	H (L)	2-methyl-~{N}-[4-[(2~{S})-2-(2-morpholin-4-ylethyl)piperidin-1-yl]sulfonylphenyl]-5-(trifluoromethyl)pyrazole-3-carboxamide	non-polymer		529.6	1	Chemie (A1EF9) PubChem (57521469)
5	I (L)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

L: -382 - -19 (UniProt: P0AEX9)

PDB	External Database	Details
Met -428	-	initiating methionine
Gly -427	-	expression tag
Ser -426	-	expression tag
Gly -425	-	expression tag
Trp -424	-	expression tag
Ser -423	-	expression tag
His -422	-	expression tag
Pro -421	-	expression tag
Gln -420	-	expression tag
Phe -419	-	expression tag
Glu -418	-	expression tag
Lys -417	-	expression tag
Gly -416	-	expression tag
Gly -415	-	expression tag
Gly -414	-	expression tag
Ser -413	-	expression tag
Gly -412	-	expression tag
Gly -411	-	expression tag
Gly -410	-	expression tag
Ser -409	-	expression tag
Gly -408	-	expression tag
Gly -407	-	expression tag
Ser -406	-	expression tag
Ala -405	-	expression tag
Trp -404	-	expression tag
Ser -403	-	expression tag
His -402	-	expression tag
Pro -401	-	expression tag
Gln -400	-	expression tag
Phe -399	-	expression tag
Glu -398	-	expression tag
Lys -397	-	expression tag
Gly -396	-	expression tag
Ser -395	-	expression tag
Ala -394	-	expression tag
Ser -393	-	expression tag
His -392	-	expression tag
His -391	-	expression tag
His -390	-	expression tag
His -389	-	expression tag
His -388	-	expression tag
His -387	-	expression tag
Gly -386	-	expression tag
Thr -385	-	expression tag
Lys -384	-	expression tag
Thr -383	-	expression tag
Gly -18	-	linker
Thr -17	-	linker
Asp -16	-	linker
Tyr -15	-	linker
Asp -14	-	linker
Ile -13	-	linker
Pro -12	-	linker
Thr -11	-	linker
Thr -10	-	linker
Leu -9	-	linker
Glu -8	-	linker
Val -7	-	linker
Leu -6	-	linker
Phe -5	-	linker
Gln -4	-	linker
Gly -3	-	linker
Pro -2	-	linker
Gly -1	-	linker
Ser 0	-	linker

L: 1 - 1465 (UniProt: Q997F0)

PDB	External Database	Details
Ser 1466	-	expression tag
Arg 1467	-	expression tag
Asp 1468	-	expression tag
Tyr 1469	-	expression tag
Lys 1470	-	expression tag
Asp 1471	-	expression tag
Asp 1472	-	expression tag
Asp 1473	-	expression tag
Asp 1474	-	expression tag
Lys 1475	-	expression tag

A, B, C, D: 86 - 449 (UniProt: P0AEX9)

PDB	External Database	Details
Met 72	-	initiating methionine
Gly 73	-	expression tag
Ser 74	-	expression tag
Ser 75	-	expression tag
His 76	-	expression tag
His 77	-	expression tag
His 78	-	expression tag
His 79	-	expression tag
His 80	-	expression tag
His 81	-	expression tag
Gly 82	-	expression tag
Thr 83	-	expression tag
Lys 84	-	expression tag
Thr 85	-	expression tag
Gly 450	-	linker
Thr 451	-	linker
Asp 452	-	linker
Tyr 453	-	linker
Asp 454	-	linker
Ile 455	-	linker
Pro 456	-	linker
Thr 457	-	linker
Thr 458	-	linker
Leu 459	-	linker
Glu 460	-	linker
Val 461	-	linker
Leu 462	-	linker
Phe 463	-	linker
Gln 464	-	linker
Gly 465	-	linker
Pro 466	-	linker
Leu 467	-	linker
Gly 468	-	linker
Ser 469	-	linker

A, B, C, D: 470 - 709 (UniProt: Q9IK91)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	499811.1
Non-Polymers*	Number of molecules	3
	Total formula weight	660.4
All*	Total formula weight	500471.5

*Water molecules are not included.