9VQL

Cryo-EM structure of the dimeric Elapor1 mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Endosome/lysosome-associated apoptosis and autophagy regulator 1	polymer	1006	110429.4	2	UniProt (A0A0A0MQC6)	Mus musculus (mice)
2	C, D, E, F (C, D, E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
3	G, H, I, J, K... (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, B: 41 - 1013 (UniProt: A0A0A0MQC6)

PDB	External Database	Details
Met 8	-	initiating methionine
Lys 9	-	expression tag
Thr 10	-	expression tag
Ile 11	-	expression tag
Ile 12	-	expression tag
Ala 13	-	expression tag
Leu 14	-	expression tag
Ser 15	-	expression tag
Tyr 16	-	expression tag
Ile 17	-	expression tag
Phe 18	-	expression tag
Cys 19	-	expression tag
Leu 20	-	expression tag
Val 21	-	expression tag
Phe 22	-	expression tag
Ala 23	-	expression tag
Asp 24	-	expression tag
Tyr 25	-	expression tag
Lys 26	-	expression tag
Asp 27	-	expression tag
Asp 28	-	expression tag
Asp 29	-	expression tag
Asp 30	-	expression tag
Lys 31	-	expression tag
Gly 32	-	expression tag
Ser 33	-	expression tag
His 34	-	expression tag
His 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
Ser 40	-	expression tag
Met 42	Thr 42	engineered mutation
Ala 47	His 47	engineered mutation
Ala 55	His 55	engineered mutation
Ala 74	His 74	engineered mutation
Ala 309	His 309	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	220858.8
Branched	Number of molecules	4
	Total formula weight	1697.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	223883.7

*Water molecules are not included.