9VQ2

Cryo-EM structure of a human flippase mutant ATP11C Q79A-CDC50A in the PtdSer-occluded E2-AlF state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phospholipid-transporting ATPase IG	polymer	1084	124403.6	1	UniProt (Q8NB49) Pfam (PF16209) Pfam (PF00122) Pfam (PF13246) Pfam (PF16212)	Homo sapiens (human)	ATPase IQ,ATPase class VI type 11C,P4-ATPase flippase complex alpha subunit ATP11C
2	B (C)	Cell cycle control protein 50A	polymer	361	40900.8	1	UniProt (Q9NV96) Pfam (PF03381)	Homo sapiens (human)	P4-ATPase flippase complex beta subunit TMEM30A,Transmembrane protein 30A
3	C (B)	alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		383.3	1	In PDB GlyTouCan (G35893LO)
4	D (A)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	1	Chemie (ALF)
5	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	F (A)	O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine	non-polymer		792.1	1	Chemie (P5S)
7	G, H, I (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
8	J (C)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 10 - 1091 (UniProt: Q8NB49)

PDB	External Database	Details
Arg 8	-	expression tag
Phe 9	-	expression tag
Trp 111	Cys 114	variant

C: 1 - 361 (UniProt: Q9NV96)

PDB	External Database	Details
Met 175	Ala 175	variant
Gln 190	Asn 190	variant
Trp 292	Ser 292	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	165304.4
Branched	Number of molecules	1
	Total formula weight	383.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1583.0
All*	Total formula weight	167270.7

*Water molecules are not included.