9VNT

Cryo-EM structure of a human flippase mutant ATP11C Q79E-CDC50A in PtdSer-occluded E2P-AlF state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phospholipid-transporting ATPase IG	polymer	1073	123168.2	1	UniProt (Q8NB49) Pfam (PF16209) Pfam (PF00122) Pfam (PF13246) Pfam (PF16212)	Homo sapiens (human)	ATPase IQ,ATPase class VI type 11C,P4-ATPase flippase complex alpha subunit ATP11C
2	B (C)	Cell cycle control protein 50A	polymer	324	37089.6	1	UniProt (Q9NV96) Pfam (PF03381)	Homo sapiens (human)	P4-ATPase flippase complex beta subunit TMEM30A,Transmembrane protein 30A
3	C (B)	alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G62182OO)
4	D (A)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	1	Chemie (ALF)
5	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	F (A)	O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine	non-polymer		792.1	1	Chemie (P5S)
7	G (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
8	H (A)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 19 - 1091 (UniProt: Q8NB49)

PDB	External Database	Details
Glu 79	Gln 82	engineered mutation
Trp 111	Cys 114	variant

C: 27 - 350 (UniProt: Q9NV96)

PDB	External Database	Details
Met 175	Ala 175	variant
Gln 190	Asn 190	variant
Trp 292	Ser 292	variant

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	160257.8
Branched	Number of molecules	1
	Total formula weight	586.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1140.6
All*	Total formula weight	161984.9

*Water molecules are not included.