9VL3

Cryo-EM structure of human lipid phosphate phosphatase 1 complexed with PO4 in nanodiscs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Phospholipid phosphatase 1	polymer	284	32126.0	4	UniProt (O14494) Pfam (PF01569)	Homo sapiens (human)	Lipid phosphate phosphohydrolase 1,PAP2-alpha,Phosphatidate phosphohydrolase type 2a,Phosphatidic acid phosphatase 2a,PAP-2a,PAP2a
2	E, J, O, T (A, B, C, D)	2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE	non-polymer		648.9	4	Chemie (LPP) PubChem (3099) PubChem (446066) PubChem (11061290)
3	F, K, P, U (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
4	G, L, Q, V (A, B, C, D)	1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine	non-polymer		760.1	4	Chemie (LBN)
5	H, M, R, W (A, B, C, D)	[(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8Z)-icosa-5,8,11,14-tetraenoate	non-polymer		1047.1	4	Chemie (PT5) PubChem (21584053) PubChem (46891800) PubChem (24742075) PubChem (25021172) PubChem (53480261) PubChem (49867532) PubChem (53731018) PubChem (74343116) PubChem (644093) PubChem (88368868) PubChem (101127471) PubChem (5497157) PubChem (101103400) PubChem (137349554) PubChem (146672970) PubChem (162802760) PubChem (177693253) PubChem (137349667) PubChem (155387720)
6	I, N, S, X (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4) PubChem (1061)

Sequence modifications

A, B, C, D: 1 - 284 (UniProt: O14494)

PDB	External Database	Details
Ala 223	His 223	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128503.9
Non-Polymers*	Number of molecules	20
	Total formula weight	11088.9
All*	Total formula weight	139592.8

*Water molecules are not included.