9VJX

Structure of the plant diacylglycerol O-acyltransferase 1 H447A mutant

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Diacylglycerol O-acyltransferase 1	polymer	528	59962.6	2	UniProt (Q9SLD2) Pfam (PF03062)	Arabidopsis thaliana (thale cress)	AtDGAT1,Protein TRIACYLGLYCEROL 1
2	C, D, E, F, K... (B, A)	OLEIC ACID	non-polymer		282.5	9	Chemie (OLA)
3	G (B)	COENZYME A	non-polymer		767.5	1	Chemie (COA)
4	H (B)	2,3-bis[[(Z)-octadec-9-enoyl]oxy]propyl (Z)-octadec-9-enoate	non-polymer		885.4	1	Chemie (A1LVF)
5	I, Q (B, A)	2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE	non-polymer		648.9	2	Chemie (LPP)
6	J, R, S, T (B, A)	[(2~{S})-2-[(~{Z})-octadec-9-enoyl]oxy-3-oxidanyl-propyl] (~{Z})-octadec-9-enoate	non-polymer		621.0	4	Chemie (A1EW5)
7	P (A)	S-{(3R,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14-dioxo-2,4,6-trioxa-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaheptadecan-17-yl} (9Z)-octadec-9-enethioate (non-preferred name)	non-polymer		1032.0	1	Chemie (3VV)
8	U, V (B, A)	water	water		18.0	6	Chemie (HOH)

Sequence modifications

B, A: 1 - 520 (UniProt: Q9SLD2)

PDB	External Database	Details
Ala 447	His 447	engineered mutation
Gly 521	-	expression tag
Pro 522	-	expression tag
His 523	-	expression tag
His 524	-	expression tag
His 525	-	expression tag
His 526	-	expression tag
His 527	-	expression tag
His 528	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	119925.3
Non-Polymers*	Number of molecules	18
	Total formula weight	9008.8
All*	Total formula weight	128934.1

*Water molecules are not included.