9VJT

Cryo-EM structure of MST-OR complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	384	41729.6	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D (E)	scFv16	polymer	266	28636.8	1		Mus musculus
5	E (R)	Mu-type opioid receptor,Oplophorus-luciferin 2-monooxygenase catalytic subunit	polymer	565	62558.4	1	UniProt (P35372) UniProt (Q9GV45)	Homo sapiens (human)	M-OR-1,MOR-1,Mu opiate receptor,Mu opioid receptor,MOP,hMOP,19kOLase
6	F (R)	Levomethadone	non-polymer		309.4	1	Chemie (A1ESG)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	conflict
Ala 203	Gly 203	conflict
Ala 245	Glu 245	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	expression tag
Gly 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Ser 351	-	expression tag
Ser 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: 1 - 368 (UniProt: P35372)

PDB	External Database	Details
Met -23	-	initiating methionine
Lys -22	-	expression tag
Thr -21	-	expression tag
Ile -20	-	expression tag
Ile -19	-	expression tag
Ala -18	-	expression tag
Leu -17	-	expression tag
Ser -16	-	expression tag
Tyr -15	-	expression tag
Ile -14	-	expression tag
Phe -13	-	expression tag
Cys -12	-	expression tag
Leu -11	-	expression tag
Val -10	-	expression tag
Phe -9	-	expression tag
Ala -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag
Gly 369	-	linker
Gly 370	-	linker
Ser 371	-	linker
Gly 372	-	linker
Gly 373	-	linker
Gly 374	-	linker
Gly 375	-	linker
Ser 376	-	linker
Gly 377	-	linker
Gly 378	-	linker
Ser 379	-	linker
Ser 380	-	linker
Ser 381	-	linker
Gly 382	-	linker
Gly 383	-	linker
Val 384	-	linker

R: 385 - 541 (UniProt: Q9GV45)

PDB	External Database	Details
Glu 388	Ala 31	conflict
Glu 395	Gln 38	conflict
Ala 399	Gly 42	conflict
Leu 402	Gln 45	conflict
Val 411	Leu 54	conflict
Leu 415	Phe 58	conflict
Asn 417	Ala 60	conflict
Ala 419	Gly 62	conflict
Arg 427	Lys 70	conflict
Ile 428	Val 71	conflict
Arg 430	Leu 73	conflict
Ala 435	Gly 78	conflict
Ile 438	Ala 81	conflict
Ala 451	Gly 94	conflict
Asp 452	Phe 95	conflict
Ala 455	Gly 98	conflict
Gln 456	Leu 99	conflict
Glu 459	Met 102	conflict
Val 460	Ile 103	conflict
Val 474	Ile 117	conflict
Pro 477	His 120	conflict
Leu 491	Ile 134	conflict
Asn 492	Asp 135	conflict
Glu 499	Pro 142	conflict
Lys 508	Gln 151	conflict
Ile 522	Tyr 165	conflict
Thr 528	Asn 171	conflict
Met 533	Leu 176	conflict
Ser 541	Gly 184	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	181199.9
Non-Polymers*	Number of molecules	1
	Total formula weight	309.4
All*	Total formula weight	181509.4

*Water molecules are not included.