9VIX

human alpha 7 nicotinic acetylcholine receptor L278A mutant in complex with L-nicotine (desensitized state)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Neuronal acetylcholine receptor subunit alpha-7	polymer	512	57574.2	5	UniProt (P36544) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	nAChR7,Nicotinic acetylcholine receptor subunit alpha-7
2	F, G, H, I, J (F, G, H, I, J)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
3	K, M, O, Q, S (A, B, C, D, E)	(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE	non-polymer		162.2	5	Chemie (NCT)
4	L, N, P, R, T (A, B, C, D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)
5	U, V, W, X, Y (A, B, C, D, E)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 502 (UniProt: P36544)

PDB	External Database	Details
Ala 278	Leu 278	engineered mutation
Gly 503	-	expression tag
Gly 504	-	expression tag
Asp 505	-	expression tag
Tyr 506	-	expression tag
Lys 507	-	expression tag
Asp 508	-	expression tag
Asp 509	-	expression tag
Asp 510	-	expression tag
Asp 511	-	expression tag
Lys 512	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	287870.9
Branched	Number of molecules	5
	Total formula weight	2122.0
Non-Polymers*	Number of molecules	10
	Total formula weight	1917.2
All*	Total formula weight	291910.1

*Water molecules are not included.