9VIT

Crystal strcture of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase CobU H49A from Akkermansia muciniphila in complex with GTP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Adenosylcobinamide kinase	polymer	202	22040.1	3	UniProt (B2UM52) Pfam (PF02283)	Akkermansia muciniphila ATCC BAA-835	Adenosylcobinamide-phosphate guanylyltransferase
2	D, G (A, C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
3	E, H (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	F (B)	TRIPHOSPHATE	non-polymer		258.0	1	Chemie (3PO)
5	I, J, K (A, B, C)	water	water		18.0	93	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 182 (UniProt: B2UM52)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 49	His 49	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	66120.4
Non-Polymers*	Number of molecules	5
	Total formula weight	1352.9
All*	Total formula weight	67473.3

*Water molecules are not included.