9VIJ

Crystal structure of fused glycerol dehydratase A177M/M158W variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, C)	Glycerol dehydrase alpha subunit,Glycerol dehydrase beta subunit	polymer	754	82617.7	2	UniProt (Q59476) UniProt (O08505)	Klebsiella pneumoniae	Glycerol dehydratase,Glycerol dehydratase medium subunit,Propanediol dehydratase,Propanediol/glycerol family dehydratase large subunit,Glycerol dehydratase medium subunit,Propanediol dehydratase,Propanediol/glycerol family dehydratase medium subunit
2	C, D (D, E)	Glycerol dehydrase gamma subunit	polymer	141	16074.3	2	UniProt (Q59475)	Klebsiella pneumoniae	Glycerol dehydratase,Glycerol dehydratase small subunit DhaB3,Glycerol dehydrogenase,Propanediol dehydratase small subunit
3	E, F (C)	COBALAMIN	non-polymer		1330.4	2	Chemie (B12)

Sequence modifications

B, C: 1 - 555 (UniProt: Q59476)

PDB	External Database	Details
Met 177	Ala 177	engineered mutation
Gln 556	-	linker
Gly 557	-	linker
Gly 558	-	linker
Ile 559	-	linker
Pro 560	-	linker
Val 561	-	linker

B, C: 562 - 754 (UniProt: O08505)

PDB	External Database	Details
Trp 718	Met 158	engineered mutation

D, E: 1 - 141 (UniProt: Q59475)

PDB	External Database	Details
Cys 51	Arg 51	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	197384.0
Non-Polymers*	Number of molecules	2
	Total formula weight	2660.7
All*	Total formula weight	200044.7

*Water molecules are not included.