9VCB

Cryo-EM structure of Shewanella putrefaciens ComEC in complex with ssDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA internalization-related competence protein ComEC/Rec2	polymer	799	89194.5	1	UniProt (A0ABX8XFP2)	Shewanella putrefaciens
2	B (B)	DNA (30-MER)	polymer	30	9178.0	1		synthetic construct
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN) PubChem (32051)

Sequence modifications

A: 1 - 774 (UniProt: A0ABX8XFP2)

PDB	External Database	Details
Met -24	-	initiating methionine
Gly -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 577	Asp 577	engineered mutation
Ala 579	Asp 579	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	98372.5
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	98437.9

*Water molecules are not included.