9VAW

Cryo-EM structure of human Neurotensin Receptor 1 (hNTSR1)-Gq (delipidated) complex in nucleotide-free C state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Neurotensin receptor type 1	polymer	436	48535.1	1	UniProt (P30989)	Homo sapiens (human)	NT-R-1,NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH
2	B (L)	JMV449	polymer	6	763.0	1		synthetic construct
3	C (A)	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	353	41365.1	1	UniProt (P50148)	Homo sapiens (human)	Guanine nucleotide-binding protein alpha-q
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39430.1	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
6	F (D)	scFv16	polymer	259	27784.9	1		Mus musculus
7	G, H, I, J, K (R, A, B, C, D)	water	water		18.0	205	Chemie (HOH)

Sequence modifications

R: 20 - 418 (UniProt: P30989)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
Gln -9	-	expression tag
Pro -8	-	expression tag
Gly -7	-	expression tag
Asn -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Ala -3	-	expression tag
Phe -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Ala 1	-	expression tag
Pro 2	-	expression tag
Asn 3	-	expression tag
Arg 4	-	expression tag
Ser 5	-	expression tag
His 6	-	expression tag
Ala 7	-	expression tag
Pro 8	-	expression tag
Asp 9	-	expression tag
His 10	-	expression tag
Asp 11	-	expression tag
Val 12	-	expression tag
Glu 13	-	expression tag
Asn 14	-	expression tag
Leu 15	-	expression tag
Tyr 16	-	expression tag
Phe 17	-	expression tag
Gln 18	-	expression tag
Gly 19	-	expression tag
Leu 85	Ala 85	engineered mutation
Leu 419	-	expression tag
Glu 420	-	expression tag
Val 421	-	expression tag
Leu 422	-	expression tag
Phe 423	-	expression tag
Gln 424	-	expression tag

A: 7 - 359 (UniProt: P50148)

PDB	External Database	Details
Gly 8	Ala 8	engineered mutation
Ser 9	Cys 9	engineered mutation
Thr 10	Cys 10	engineered mutation
Ala 13	Glu 13	engineered mutation
Asp 15	Ala 15	engineered mutation
Ala 17	Glu 17	engineered mutation
Val 19	Arg 19	engineered mutation
Glu 20	Arg 20	engineered mutation
Arg 21	Ile 21	engineered mutation
Ser 22	Asn 22	engineered mutation
Lys 23	Asp 23	engineered mutation
Met 24	Glu 24	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Ile 159	Thr 159	engineered mutation

C: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	165723.2
All*	Total formula weight	165723.2

*Water molecules are not included.