9V9Y
Cryo-EM structure of the ncPRC1.4 complex containing one RNF2-BMI1 and one RYBP bound to the H2AK119ub-modified nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3 | polymer | 136 | 15435.1 | 2 | UniProt (A0A310TTQ1) | Xenopus laevis (African clawed frog) | |
| 2 | B, F (B, F) | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) | Xenopus laevis (African clawed frog) | |
| 3 | C, G (C, G) | Histone H2A | polymer | 130 | 14083.4 | 2 | UniProt (Q6AZJ8) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (D, H) | Histone H2B | polymer | 126 | 13939.2 | 2 | UniProt (A0A8J0TWI5) | Xenopus laevis (African clawed frog) | |
| 5 | I (I) | DNA (131-MER) | polymer | 131 | 40237.6 | 1 | Homo sapiens | ||
| 6 | J (J) | DNA (132-MER) | polymer | 132 | 40942.1 | 1 | Homo sapiens | ||
| 7 | K (M) | Polycomb complex protein BMI-1 | polymer | 326 | 37006.1 | 1 | UniProt (P35226) | Homo sapiens (human) | Polycomb group RING finger protein 4,RING finger protein 51 |
| 8 | L (N) | E3 ubiquitin-protein ligase RING2 | polymer | 336 | 37706.4 | 1 | UniProt (Q99496) | Homo sapiens (human) | Huntingtin-interacting protein 2-interacting protein 3,HIP2-interacting protein 3,Protein DinG,RING finger protein 1B,RING1b,RING finger protein 2,RING finger protein BAP-1,RING-type E3 ubiquitin transferase RING2 |
| 9 | M (K) | Ubiquitin | polymer | 76 | 8622.9 | 1 | UniProt (P0CG47) | Homo sapiens (human) | |
| 10 | N (L) | RING1 and YY1-binding protein | polymer | 228 | 24863.6 | 1 | UniProt (Q8N488) | Homo sapiens (human) | Apoptin-associating protein 1,APAP-1,Death effector domain-associated factor,DED-associated factor,YY1 and E4TF1-associated factor 1 |
| 11 | O, P, Q, R, S (M, N, L) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) PubChem (32051) |
Sequence modifications
C, G: 0 - 129 (UniProt: Q6AZJ8)
K: 1 - 76 (UniProt: P0CG47)
| PDB | External Database | Details |
|---|---|---|
| Cys 119 | Lys 120 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Cys 76 | Gly 228 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 299083.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 327.0 | |
| All* | Total formula weight | 299410.1 |
