9V9U

Cryo-EM structure of the ncPRC1.4 complex containing two RNF2-BMI1 bound to the H2AK119ubH2BK120ub-modified nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3	polymer	136	15435.1	2	UniProt (A0A310TTQ1)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A	polymer	130	14083.4	2	UniProt (Q6AZJ8)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B	polymer	126	13913.2	2	UniProt (A0A8J0TWI5)	Xenopus laevis (African clawed frog)
5	I (I)	DNA (132-MER)	polymer	132	40499.8	1		Homo sapiens
6	J (J)	DNA (132-MER)	polymer	132	40980.1	1		Homo sapiens
7	K, N (M, K)	Polycomb complex protein BMI-1	polymer	326	37006.1	2	UniProt (P35226)	Homo sapiens (human)	Polycomb group RING finger protein 4,RING finger protein 51
8	L, O (N, L)	E3 ubiquitin-protein ligase RING2	polymer	336	37706.4	2	UniProt (Q99496)	Homo sapiens (human)	Huntingtin-interacting protein 2-interacting protein 3,HIP2-interacting protein 3,Protein DinG,RING finger protein 1B,RING1b,RING finger protein 2,RING finger protein BAP-1,RING-type E3 ubiquitin transferase RING2
9	M, P (O, P)	Ubiquitin	polymer	76	8622.9	2	UniProt (P0CG47)	Homo sapiens (human)
10	Q, R, S, T, U... (M, N, K, L)	ZINC ION	non-polymer		65.4	8	Chemie (ZN) PubChem (32051)

Sequence modifications

C, G: 0 - 129 (UniProt: Q6AZJ8)

PDB	External Database	Details
Cys 119	Lys 120	engineered mutation

D, H: -3 - 122 (UniProt: A0A8J0TWI5)

PDB	External Database	Details
Cys 117	Lys 121	engineered mutation

O, P: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Cys 76	Gly 228	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	357802.9
Non-Polymers*	Number of molecules	8
	Total formula weight	523.3
All*	Total formula weight	358326.2

*Water molecules are not included.