9V6O
Cryo-EM structure of human kappa opioid receptor - G protein signaling complex bound with nalfurafine.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37728.2 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (R) | Kappa-type opioid receptor | polymer | 341 | 38707.8 | 1 | UniProt (P41145) Pfam (PF00001) | Homo sapiens (human) | |
| 5 | E (S) | scFv16 | polymer | 255 | 27318.4 | 1 | Homo sapiens | ||
| 6 | F (R) | nalfurafine | non-polymer | 476.6 | 1 | Chemie (IVB) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: 54 - 380 (UniProt: P41145)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 48 | - | expression tag |
| Pro 49 | - | expression tag |
| Gly 50 | - | expression tag |
| Ser 51 | - | expression tag |
| Thr 52 | - | expression tag |
| Ser 53 | - | expression tag |
| Leu 135 | Ile 135 | engineered mutation |
| Glu 381 | - | expression tag |
| Phe 382 | - | expression tag |
| Leu 383 | - | expression tag |
| Glu 384 | - | expression tag |
| Val 385 | - | expression tag |
| Leu 386 | - | expression tag |
| Phe 387 | - | expression tag |
| Gln 388 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 152030.5 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 476.6 | |
| All* | Total formula weight | 152507.1 |
