9V33

Calypso/Asx/NCP-ub complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3	polymer	136	15435.1	2	UniProt (A0A310TTQ1)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A	polymer	132	14297.7	2	UniProt (Q6AZJ8)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B	polymer	123	13655.9	2	UniProt (A0A8J0U496)	Xenopus laevis (African clawed frog)
5	I (I)	DNA (147-MER)	polymer	147	45178.8	1		Escherichia coli
6	J (J)	DNA (147-MER)	polymer	147	45570.0	1		Escherichia coli
7	K, N (K, N)	Ubiquitin carboxyl-terminal hydrolase calypso	polymer	512	55928.8	2	UniProt (Q7K5N4)	Drosophila melanogaster (fruit fly)	BRCA1-associated protein 1 homolog,BAP1 homolog,Polycomb group protein calypso
8	L, O (L, O)	Polycomb group protein Asx	polymer	340	37098.0	2	UniProt (Q9V727)	Drosophila melanogaster (fruit fly)	Protein additional sex combs
9	M (M)	Polyubiquitin-B	polymer	88	10001.4	1	UniProt (P0CG47)	Homo sapiens (human)

Sequence modifications

C, G: 0 - 129 (UniProt: Q6AZJ8)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag

D, H: 1 - 122 (UniProt: A0A8J0U496)

PDB	External Database	Details
Met 0	-	initiating methionine

K, N: 1 - 471 (UniProt: Q7K5N4)

PDB	External Database	Details
Met -40	-	initiating methionine
Trp -39	-	expression tag
Ser -38	-	expression tag
His -37	-	expression tag
Pro -36	-	expression tag
Gln -35	-	expression tag
Phe -34	-	expression tag
Glu -33	-	expression tag
Lys -32	-	expression tag
Gly -31	-	expression tag
Gly -30	-	expression tag
Gly -29	-	expression tag
Ser -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Trp -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Pro -16	-	expression tag
Gln -15	-	expression tag
Phe -14	-	expression tag
Glu -13	-	expression tag
Lys -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

M: 1 - 75 (UniProt: P0CG47)

PDB	External Database	Details
Gly -11	-	expression tag
Pro -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Leu -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Cys 76	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	396370.1
All*	Total formula weight	396370.1

*Water molecules are not included.