9UZ7
Cryo-EM structure of the nucleosome core particle with site-specific DNA-histone crosslinking
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Histone H3.1 | polymer | 135 | 15247.8 | 1 | UniProt (P68431) Pfam (PF00125) | Homo sapiens (human) | Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l |
| 2 | B (B) | Histone H3.1 | polymer | 135 | 15304.9 | 1 | UniProt (P68431) Pfam (PF00125) | Homo sapiens (human) | Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l |
| 3 | C, D (C, D) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62805) Pfam (PF15511) | Homo sapiens (human) | |
| 4 | E, F (E, F) | Histone H2A type 1-B/E | polymer | 129 | 14034.4 | 2 | UniProt (P04908) Pfam (PF00125) Pfam (PF16211) | Homo sapiens (human) | Histone H2A.2,Histone H2A/a,Histone H2A/m |
| 5 | G, H (G, H) | Histone H2B type 1-J | polymer | 125 | 13804.0 | 2 | UniProt (P06899) Pfam (PF00125) | Homo sapiens (human) | Histone H2B.1,Histone H2B.r,H2B/r |
| 6 | I (I) | DNA (145-MER) | polymer | 145 | 45007.7 | 1 | synthetic construct | ||
| 7 | J (J) | DNA (145-MER) | polymer | 145 | 44505.4 | 1 | synthetic construct |
Sequence modifications
A: 1 - 135 (UniProt: P68431)
B: 1 - 135 (UniProt: P68431)
| PDB | External Database | Details |
|---|---|---|
| Ser 96 | Cys 97 | engineered mutation |
| Ser 110 | Cys 111 | engineered mutation |
| Cys 115 | Lys 116 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 96 | Cys 97 | engineered mutation |
| Ser 110 | Cys 111 | engineered mutation |
| Ycm 115 | Lys 116 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 198268.9 | |
| All* | Total formula weight | 198268.9 |
