9UYN

Cryo-EM structure of the G protein-coupled receptor 1 (GPR1) bound to beta-arrestin 1 in ligand-free state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Chemerin-like receptor 2,Vasopressin V2 receptor	polymer	414	48035.9	1	UniProt (P46091) UniProt (P30518) Pfam (PF00001)	Homo sapiens (human)	Chemerin chemokine-like receptor 2,Chemokine-like receptor 2,G-protein coupled receptor 1,V2R,AVPR V2,Antidiuretic hormone receptor,Renal-type arginine vasopressin receptor
2	B (A)	Beta-arrestin-1	polymer	376	42107.1	1	UniProt (P49407) Pfam (PF00339) Pfam (PF02752)	Homo sapiens (human)	Arrestin beta-1,Non-visual arrestin-2
3	C (B)	Nanobody 32	polymer	120	13238.7	1		Escherichia phage EcSzw-2
4	D (C)	Single-chain fragment variable 30 (scFv30)	polymer	245	26157.9	1		Homo sapiens (human)
5	E (R)	PALMITOLEIC ACID	non-polymer		254.4	1	Chemie (PAM)

Sequence modifications

R: 2 - 1342 (UniProt: P46091)

PDB	External Database	Details
Met -62	-	initiating methionine
Lys -61	-	expression tag
Thr -60	-	expression tag
Ile -59	-	expression tag
Ile -58	-	expression tag
Ala -57	-	expression tag
Leu -56	-	expression tag
Ser -55	-	expression tag
Tyr -54	-	expression tag
Ile -53	-	expression tag
Phe -52	-	expression tag
Cys -51	-	expression tag
Leu -50	-	expression tag
Val -49	-	expression tag
Phe -48	-	expression tag
Ala -47	-	expression tag
Gly -46	-	expression tag
Ser -45	-	expression tag
Trp -44	-	expression tag
Ser -43	-	expression tag
His -42	-	expression tag
Pro -41	-	expression tag
Gln -40	-	expression tag
Phe -39	-	expression tag
Glu -38	-	expression tag
Lys -37	-	expression tag
Gly -36	-	expression tag
Ser -35	-	expression tag
Gly -34	-	expression tag
Ala -33	-	expression tag
Gly -32	-	expression tag
Ala -31	-	expression tag
Ser -30	-	expression tag
Ala -29	-	expression tag
Gly -28	-	expression tag
Ser -27	-	expression tag
Trp -26	-	expression tag
Ser -25	-	expression tag
His -24	-	expression tag
Pro -23	-	expression tag
Gln -22	-	expression tag
Phe -21	-	expression tag
Glu -20	-	expression tag
Lys -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Glu -8	-	expression tag
Phe -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Pro 1	-	expression tag
Cys 143	Val 143	engineered mutation

R: 1343 - 1371 (UniProt: P30518)
A: 1 - 376 (UniProt: P49407)

PDB	External Database	Details
Ala 59	Cys 59	engineered mutation
Ser 125	Cys 125	engineered mutation
Ile 140	Cys 140	engineered mutation
Val 150	Cys 150	engineered mutation
Glu 169	Arg 169	engineered mutation
Val 242	Cys 242	engineered mutation
Cys 249	Tyr 249	engineered mutation
Val 251	Cys 251	engineered mutation
Ser 269	Cys 269	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	129539.5
Non-Polymers*	Number of molecules	1
	Total formula weight	254.4
All*	Total formula weight	129793.9

*Water molecules are not included.