9UXZ

Crystal structure of the P450BM3 triple mutant F87A/L215W/H408W from Priestia megaterium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Bifunctional cytochrome P450/NADPH--P450 reductase	polymer	465	53473.9	2	UniProt (P14779)	Priestia megaterium (strain ATCC 14581 / DSM 32 / CCUG 1817 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / NRRL B-14308 / VKM B-512 / Ford 19) (Bacillus megaterium)	Cytochrome P450(BM-3),Cytochrome P450BM-3,Fatty acid monooxygenase,Flavocytochrome P450 BM3
2	C, D (D, E)	I7X-PHE-PHE	polymer	3	476.6	2		synthetic construct
3	E, M (A, B)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
4	F (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL) PubChem (753)
5	G, H, I, J, K (A)	METHANOL	non-polymer		32.0	5	Chemie (MOH) PubChem (887)
6	L, N (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
7	O, P, Q, R (A, B, D, E)	water	water		18.0	1226	Chemie (HOH)

Sequence modifications

A, B: 0 - 455 (UniProt: P14779)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 87	Phe 88	engineered mutation
Trp 215	Leu 216	engineered mutation
Trp 408	His 409	engineered mutation
Leu 456	-	expression tag
Glu 457	-	expression tag
His 458	-	expression tag
His 459	-	expression tag
His 460	-	expression tag
His 461	-	expression tag
His 462	-	expression tag
His 463	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	107900.9
Non-Polymers*	Number of molecules	10
	Total formula weight	1533.9
All*	Total formula weight	109434.8

*Water molecules are not included.