9UU4

Cryo-EM structure of the maize CER6-GL2 complex (inactive C222A mutant) bound with malonyl-CoA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	Protein ECERIFERUM 26-like	polymer	434	46865.0	2	UniProt (A0A804MAL4) Pfam (PF02458)	Zea mays (Maize)	GL2
2	C, D (C, B)	3-ketoacyl-CoA synthase	polymer	500	56381.4	2	UniProt (B4G0N2) Pfam (PF08392) Pfam (PF08541)	Zea mays (Maize)	CER6
3	E, F (C, B)	MALONYL-COENZYME A	non-polymer		853.6	2	Chemie (MLC)

Sequence modifications

A, D: 1 - 426 (UniProt: A0A804MAL4)

PDB	External Database	Details
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag

C, B: 1 - 494 (UniProt: B4G0N2)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 222	Cys 222	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	206492.7
Non-Polymers*	Number of molecules	2
	Total formula weight	1707.2
All*	Total formula weight	208199.9

*Water molecules are not included.