9UPM

Cryo-EM structure of macaque red cone pigment with Q114N mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	LW opsin,Soluble cytochrome b562	polymer	452	49925.6	1	UniProt (Q9XSX4) UniProt (P0ABE7)	Macaca fascicularis (crab-eating macaque)	Cytochrome b-562
2	B (A)	RETINAL	non-polymer		284.4	1	Chemie (RET) PubChem (638015) PubChem (5381765)

Sequence modifications

A: 1 - 250 (UniProt: Q9XSX4)

PDB	External Database	Details
Asn 114	Gln 114	engineered mutation

A: 251 - 290 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 257	Met 29	engineered mutation

A: 296 - 338 (UniProt: P0ABE7)

PDB	External Database	Details
Gly 291	-	linker
Ser 292	-	linker
Gly 293	-	linker
Ser 294	-	linker
Gly 295	-	linker
Ile 331	Arg 120	engineered mutation
Ile 335	His 124	engineered mutation
Gly 339	-	linker

A: 340 - 446 (UniProt: Q9XSX4)

PDB	External Database	Details
Leu 447	-	expression tag
Glu 448	-	expression tag
Val 449	-	expression tag
Leu 450	-	expression tag
Phe 451	-	expression tag
Gln 452	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	49925.6
Non-Polymers*	Number of molecules	1
	Total formula weight	284.4
All*	Total formula weight	50210.0

*Water molecules are not included.