9ULS

CryoEM structure of the T.thermophilus transcription initiation complex bound to Up4A-C and Up4A, -1 dA in the template DNA strand

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	315	35056.2	2	UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Thermus thermophilus HB8	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1119	125436.5	1	UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560)	Thermus thermophilus HB8	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1524	170997.4	1	UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Thermus thermophilus HB8	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	99	11533.3	1	UniProt (Q8RQE7) Pfam (PF01192)	Thermus thermophilus HB8	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	RNA polymerase sigma factor SigA	polymer	444	50900.6	1	UniProt (Q5SKW1) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Thermus thermophilus HB8
6	G (G)	template DNA strand	polymer	48	14855.6	1		Thermus thermophilus HB8
7	H (H)	non-template DNA strand	polymer	48	14863.5	1		Thermus thermophilus HB8
8	I, L, O, P, Q (A, C, D)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG) PubChem (888)
9	J (B)	CHAPSO	non-polymer		631.9	1	Chemie (1N7) PubChem (16402373) PubChem (44119969) PubChem (44660322) PubChem (23636791) PubChem (23639399) PubChem (122146) PubChem (53393747) PubChem (50987332) PubChem (71464051) PubChem (3253739) PubChem (71305076) PubChem (90479263) PubChem (96545191) PubChem (124745618) PubChem (124901558) PubChem (124905421) PubChem (125027410) PubChem (10009199) PubChem (96327541) PubChem (96327542) PubChem (96327543) PubChem (96327544) PubChem (96545192) PubChem (102565431) PubChem (121392376) PubChem (122130148) PubChem (9895487) PubChem (144605089) PubChem (124901556) PubChem (124901561) PubChem (124901563) PubChem (124905422) PubChem (125027411) PubChem (129537355) PubChem (129537356) PubChem (145705472) PubChem (146030291) PubChem (146159852)
10	K, S (C, D)	P1-(5'-Adenosyl) P4-(5'-uridyl) tetraphosphate	non-polymer		813.3	2	Chemie (A1L89) PubChem (53462832) PubChem (165888) PubChem (9875771) PubChem (10284182) PubChem (11578689) PubChem (172419422) PubChem (161085050)
11	M, N (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
12	R (D)	CYTIDINE-5'-MONOPHOSPHATE	non-polymer		323.2	1	Chemie (C5P)
13	T, U (C, D)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

F: 1 - 423 (UniProt: Q5SKW1)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	458699.3
Non-Polymers*	Number of molecules	11
	Total formula weight	2834.1
All*	Total formula weight	461533.4

*Water molecules are not included.