9UJL
CryoEM structure of the T.thermophilus transcription initiation complex in the presence of Ap4A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (H) | non-template DNA strand | polymer | 48 | 14863.5 | 1 | Thermus thermophilus HB8 | ||
| 2 | B (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1524 | 170997.4 | 1 | UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Thermus thermophilus HB8 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 3 | C (G) | template DNA strand | polymer | 48 | 14831.5 | 1 | Thermus thermophilus HB8 | ||
| 4 | D (F) | RNA polymerase sigma factor SigA | polymer | 444 | 50900.6 | 1 | UniProt (Q5SKW1) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Thermus thermophilus HB8 | |
| 5 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 99 | 11533.3 | 1 | UniProt (Q8RQE7) Pfam (PF01192) | Thermus thermophilus HB8 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 6 | F (C) | DNA-directed RNA polymerase subunit beta | polymer | 1119 | 125436.5 | 1 | UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Thermus thermophilus HB8 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 7 | G, H (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 315 | 35056.2 | 2 | UniProt (Q5SHR6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Thermus thermophilus HB8 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 8 | I, J (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 9 | K, L, M (D, A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 10 | N (B) | CHAPSO | non-polymer | 631.9 | 1 | Chemie (1N7) | |||
| 11 | O (D) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
F: 1 - 423 (UniProt: Q5SKW1)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | initiating methionine |
| Gly -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Ser -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Val -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Arg -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Met 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 458675.3 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 835.6 | |
| All* | Total formula weight | 459510.9 |
