9UHU

Cryo-EM structure of the human adenosine A2A receptor in ligand free state resolved using the fusion/crosslinking strategy

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Adenosine receptor A2a	polymer	332	36790.6	1	UniProt (P29274) Pfam (PF00001)	Homo sapiens (human)
2	B (B)	Green fluorescent protein	polymer	196	22388.3	1	UniProt (A0A059PIQ0)	Aequorea victoria
3	C (C)	GFP-clamp	polymer	294	31064.6	1		synthetic construct

Sequence modifications

A: 2 - 208 (UniProt: P29274)
A: 225 - 318 (UniProt: P29274)

PDB	External Database	Details
Gly 1001	-	linker
His 1002	-	linker
Tyr 1003	-	linker
Leu 1004	-	linker
Ser 1005	-	linker
Thr 1006	-	linker
Gln 1007	-	linker
Thr 1008	-	linker
Ser 1009	-	linker
Leu 1010	-	linker
Ser 1011	-	linker
Lys 1012	-	linker
Asp 1013	-	linker
Leu 1014	-	linker
Asn 1015	-	linker
Glu 1016	-	linker
Lys 1017	-	linker
Arg 1018	-	linker
Asp 1019	-	linker
His 1020	-	linker
Met 1021	-	linker
Val 1022	-	linker
Leu 1023	-	linker
Leu 1024	-	linker
Glu 1025	-	linker
Phe 1026	-	linker
Val 1027	-	linker
Thr 1028	-	linker
Ala 1029	-	linker
Ala 1030	-	linker
Gly 1031	-	linker

B: 4 - 198 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Thr 3	-	expression tag
Ile 18	Val 16	conflict
Arg 32	Ser 30	conflict
Tyr 41	Asn 39	conflict
Cro 68	Thr 65	conflict
-	Tyr 66	deletion
-	Gly 67	deletion
Ser 74	Ala 72	conflict
Arg 82	Gln 80	conflict
Tyr 101	Ser 99	conflict
Met 155	Thr 153	conflict
Asn 160	Lys 158	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	90243.5
All*	Total formula weight	90243.5

*Water molecules are not included.