Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

9UFE

Cryo-EM structure of the tubular mastigoneme (the central tube) from golden algae 2.17 angstrom resolution

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G, M (EA, EB, EC)	OCM5	polymer	368	40219.9	3		Ochromonas danica
2	B, H, N (FA, FB, FC)	OCM6	polymer	723	78780.8	3		Ochromonas danica
3	C, I, O (DA, DB, DC)	Tubular mastigoneme protein	polymer	209	22290.1	3	UniProt (C4B7Q8)	Ochromonas danica
4	D, J, P (CA, CB, CC)	OCM3	polymer	349	36806.3	3		Ochromonas danica
5	E, K, Q (AA, AB, AC)	Tubular mastigoneme protein	polymer	748	83365.7	3	UniProt (A6P4C0)	Ochromonas danica
6	F, L, R (BA, BB, BC)	Tubular mastigoneme protein	polymer	366	40980.4	3	UniProt (C4B7Q7)	Ochromonas danica
7	AB, JA, S (aK, aF, aA)	beta-D-xylopyranose-(1-2)-beta-D-xylopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)][2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)][beta-D-xylopyranose-(1-2)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)][alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		2362.1	3	In PDB
8	BB, KA, T (aL, aG, aB)	alpha-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-D-mannopyranose-(1-2)-beta-D-xylopyranose-(1-6)][beta-D-xylopyranose-(1-3)]alpha-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-[beta-D-xylopyranose-(1-3)]alpha-D-mannopyranose-(1-6)][beta-D-xylopyranose-(1-2)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		2656.4	3	In PDB
9	CB, LA, U (aM, aH, aC)	alpha-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-4)]2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-D-xylopyranose-(1-2)-beta-D-xylopyranose-(1-6)][beta-D-glucopyranose-(1-3)]alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)][beta-D-xylopyranose-(1-2)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)][alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		2686.4	3	In PDB
10	DB, MA, V (aN, aI, aD)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1559.4	3	In PDB GlyTouCan (G04216GB)
11	EB, NA, W (aO, aJ, aE)	beta-D-xylopyranose-(1-2)-beta-D-xylopyranose-(1-6)-[beta-D-xylopyranose-(1-3)][2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-[beta-D-xylopyranose-(1-3)]alpha-D-mannopyranose-(1-6)][beta-D-xylopyranose-(1-2)]alpha-D-mannopyranose-(1-6)-[beta-D-mannopyranose-(1-2)][alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		2464.2	3	In PDB
12	FB, OA, X (bG, bD, bA)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1559.4	3	In PDB GlyTouCan (G37135JQ)
13	GB, PA, Y (bH, bE, bB)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	3	In PDB GlyTouCan (G10756ZZ)
14	HB, QA, Z (bI, bF, bC)	beta-D-xylopyranose-(1-6)-alpha-D-mannopyranose-(1-3)-[alpha-D-xylopyranose-(1-2)][alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[beta-D-mannopyranose-(1-2)][alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1661.5	3	In PDB
15	AA, IB, RA (cA, cC, cB)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-xylopyranose	branched		636.6	3	In PDB
16	BA, JB, SA (dA, dC, dB)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-xylopyranose	branched		1123.0	3	In PDB
17	CA, FA, GA, KB, NB... (eA, fC, fD, eE, fM...)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1883.7	9	In PDB GlyTouCan (G60230HH)
18	DA, LB, UA (eB, eF, eD)	alpha-D-xylopyranose-(1-2)-beta-D-xylopyranose-(1-6)-[beta-D-glucopyranose-(1-3)][2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-[beta-D-xylopyranose-(1-3)]alpha-D-mannopyranose-(1-6)][beta-D-xylopyranose-(1-2)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		2332.1	3	In PDB
19	EA, HA, MB, PB, VA... (fB, fE, fL, fO, fG...)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1721.5	6	In PDB GlyTouCan (G89864BN)
20	IA, QB, ZA (fF, fP, fK)	alpha-D-xylopyranose-(1-2)-beta-D-xylopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)]alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-[alpha-D-xylopyranose-(1-3)]alpha-D-mannopyranose-(1-6)][beta-D-xylopyranose-(1-2)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)][alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		2332.1	3	In PDB
21	AC, AD, BC, BD, BE... (FA, FB, FC, CA, CB...)	CALCIUM ION	non-polymer		40.1	55	Chemie (CA) PubChem (271)
22	SB, UC, VB, WD, XC... (EA, EB, FA, EC, FB...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG) PubChem (24139)
23	AE, FC, GC, HC, HD... (FC, AA, AB, AC, BA...)	PHOSPHONATE	non-polymer		80.0	21	Chemie (2PO) PubChem (406)
24	DC, FD, HE (DA, DB, DC)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL) PubChem (312)
25	AF, BF, CF, DF, EF... (DA, CA, AA, BA, EB...)	water	water		18.0	6687	Chemie (HOH)

Sequence modifications

DA, DB, DC: 1 - 209 (UniProt: C4B7Q8)

PDB	External Database	Details
Ser 106	Pro 106	conflict

BA, BB, BC: 1 - 366 (UniProt: C4B7Q7)

PDB	External Database	Details
Gly 72	Val 72	conflict
Asp 112	Ala 112	conflict

Sequence viewer

Contents of the asymmetric unit

Polymers	Number of chains	18
Polymers	Total formula weight	907329.2
Branched	Number of molecules	51
Branched	Total formula weight	94133.8
Non-Polymers*	Number of molecules	85
Non-Polymers*	Total formula weight	5317.5
All*	Total formula weight	1006780.5

*Water molecules are not included.

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)