9UDF
Cryo-EM structure of Na+-translocating NADH-ubiquinone oxidoreductase NqrB-G141A mutant from Vibrio cholerae reduced by NADH, with bound korormicin A, shifted state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Na(+)-translocating NADH-quinone reductase subunit A | polymer | 446 | 48680.7 | 1 | UniProt (A5F5X1) | Vibrio cholerae O395 | Na(+)-NQR subunit A,Na(+)-translocating NQR subunit A,NQR complex subunit A,NQR-1 subunit A |
| 2 | B (B) | Na(+)-translocating NADH-quinone reductase subunit B | polymer | 415 | 45404.9 | 1 | UniProt (A5F5X0) | Vibrio cholerae O395 | Na(+)-NQR subunit B,Na(+)-translocating NQR subunit B,NQR complex subunit B,NQR-1 subunit B |
| 3 | C (C) | Na(+)-translocating NADH-quinone reductase subunit C | polymer | 257 | 27652.3 | 1 | UniProt (A5F5Y7) | Vibrio cholerae O395 | Na(+)-NQR subunit C,Na(+)-translocating NQR subunit C,NQR complex subunit C,NQR-1 subunit C |
| 4 | D (D) | Na(+)-translocating NADH-quinone reductase subunit D | polymer | 210 | 22853.2 | 1 | UniProt (A5F5Y6) | Vibrio cholerae O395 | Na(+)-NQR subunit D,Na(+)-translocating NQR subunit D,NQR complex subunit D,NQR-1 subunit D |
| 5 | E (E) | Na(+)-translocating NADH-quinone reductase subunit E | polymer | 198 | 21481.7 | 1 | UniProt (A5F5Y5) | Vibrio cholerae O395 | Na(+)-NQR subunit E,Na(+)-translocating NQR subunit E,NQR complex subunit E,NQR-1 subunit E |
| 6 | F (F) | Na(+)-translocating NADH-quinone reductase subunit F | polymer | 414 | 45942.4 | 1 | UniProt (A5F5Y4) | Vibrio cholerae O395 | Na(+)-NQR subunit F,Na(+)-translocating NQR subunit F,NQR complex subunit F,NQR-1 subunit F |
| 7 | G, M (B, C) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 2 | Chemie (FMN) | |||
| 8 | H (B) | RIBOFLAVIN | non-polymer | 376.4 | 1 | Chemie (RBF) | |||
| 9 | I, K (B) | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine | non-polymer | 744.0 | 2 | Chemie (PEE) | |||
| 10 | J, O (B, D) | DODECYL-BETA-D-MALTOSIDE | non-polymer | 510.6 | 2 | Chemie (LMT) | |||
| 11 | L (B) | Korormicin | non-polymer | 433.6 | 1 | Chemie (IQT) | |||
| 12 | N (C) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 13 | P, Q (D, F) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 14 | R (F) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 1 | Chemie (FAD) | |||
| 15 | S, T (A, B) | water | water | 18.0 | 17 | Chemie (HOH) |
Sequence modifications
B: 1 - 415 (UniProt: A5F5X0)
F: 1 - 408 (UniProt: A5F5Y4)
| PDB | External Database | Details |
|---|---|---|
| Ala 141 | Gly 141 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| His 409 | - | expression tag |
| His 410 | - | expression tag |
| His 411 | - | expression tag |
| His 412 | - | expression tag |
| His 413 | - | expression tag |
| His 414 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 212015.2 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 5409.2 | |
| All* | Total formula weight | 217424.4 |
