9UCL

CryoEM structure of IgV domain of human Nectin-4 with Trimbody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	H3-PrAC-5350A,2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase	polymer	332	36127.9	3	UniProt (Q9WXS1) Pfam (PF01081)	synthetic construct
2	D, E, F (D, E, F)	N4-nanobody-TAIL	polymer	251	27671.3	3		synthetic construct
3	G, H, I (G, H, I)	Nectin-4	polymer	123	13566.1	3	UniProt (Q96NY8) Pfam (PF07686)	Homo sapiens (human)	Ig superfamily receptor LNIR,Nectin cell adhesion molecule 4,Poliovirus receptor-related protein 4

Sequence modifications

A, B, C: 1 - 117 (PDB: 9UCL)
A, B, C: 118 - 321 (UniProt: Q9WXS1)

PDB	External Database	Details
Ile 141	Lys 25	conflict
Val 145	Leu 29	conflict
Ala 149	Glu 33	conflict
Ala 170	Glu 54	conflict
Val 173	Phe 57	conflict
Ala 322	-	expression tag
Ala 323	-	expression tag
Ala 324	-	expression tag
Leu 325	-	expression tag
Glu 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag

G, H, I: 2 - 116 (UniProt: Q96NY8)

PDB	External Database	Details
Met 1	-	initiating methionine
Glu 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
His 121	-	expression tag
His 122	-	expression tag
His 123	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	232095.8
All*	Total formula weight	232095.8

*Water molecules are not included.