9UAM

CysLTR2-LTC4-miniGq complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Beta-2 adrenergic receptor,Cysteinyl leukotriene receptor 2,Oplophorus-luciferin 2-monooxygenase catalytic subunit	polymer	593	66958.7	1	UniProt (P07550) UniProt (Q9NS75)	Homo sapiens (human)	Beta-2 adrenoreceptor,Beta-2 adrenoceptor,CysLTR2,G-protein coupled receptor GPCR21,hGPCR21,G-protein coupled receptor HG57,HPN321
2	B (B)	miniGq	polymer	246	28114.9	1		Homo sapiens
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	384	41729.6	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
4	D (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	67	7432.6	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
5	E (E)	scFv16	polymer	265	28551.7	1		Homo sapiens
6	F (A)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	1	Chemie (Y01)
7	G (A)	(5~{S},6~{R},7~{E},9~{E},11~{Z},14~{Z})-6-[(2~{R})-2-[[(4~{S})-4-azanyl-5-oxidanyl-5-oxidanylidene-pentanoyl]amino]-3-( 2-hydroxy-2-oxoethylamino)-3-oxidanylidene-propyl]sulfanyl-5-oxidanyl-icosa-7,9,11,14-tetraenoic acid	non-polymer		625.8	1	Chemie (LTX)
8	H, I (A)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)

Sequence modifications

A: -37 - -9 (UniProt: P07550)

PDB	External Database	Details
Met -77	-	initiating methionine
Ala -76	-	expression tag
Thr -75	-	expression tag
Met -74	-	expression tag
Asp -73	-	expression tag
Ser -72	-	expression tag
Lys -71	-	expression tag
Gly -70	-	expression tag
Ser -69	-	expression tag
Ser -68	-	expression tag
Gln -67	-	expression tag
Lys -66	-	expression tag
Gly -65	-	expression tag
Ser -64	-	expression tag
Arg -63	-	expression tag
Leu -62	-	expression tag
Leu -61	-	expression tag
Leu -60	-	expression tag
Leu -59	-	expression tag
Leu -58	-	expression tag
Val -57	-	expression tag
Val -56	-	expression tag
Ser -55	-	expression tag
Asn -54	-	expression tag
Leu -53	-	expression tag
Leu -52	-	expression tag
Leu -51	-	expression tag
Cys -50	-	expression tag
Gln -49	-	expression tag
Gly -48	-	expression tag
Val -47	-	expression tag
Val -46	-	expression tag
Ser -45	-	expression tag
Asp -44	-	expression tag
Tyr -43	-	expression tag
Lys -42	-	expression tag
Asp -41	-	expression tag
Asp -40	-	expression tag
Asp -39	-	expression tag
Asp -38	-	expression tag
Asn -16	Asp 23	conflict
Gln -12	Glu 27	conflict
Glu -8	-	linker
Asn -7	-	linker
Leu -6	-	linker
Tyr -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Val -1	-	linker
Asp 0	-	linker

A: 1 - 346 (UniProt: Q9NS75)
A: 347 - 515 (PDB: 9UAM)
C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	expression tag
Gly 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Ser 351	-	expression tag
Ser 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	172787.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1885.8
All*	Total formula weight	174673.3

*Water molecules are not included.