9U9K

Cryo-EM structure of hemi-methylated DNA-bound Tetrahymena DNA methyltransferase complex MTA1c (MTA9-B)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	mRNA m(6)A methyltransferase	polymer	378	43368.9	1	UniProt (Q22GC0) Pfam (PF05063)	Tetrahymena thermophila SB210
2	B (B)	Methyltransferase MT, putative	polymer	357	41270.0	1	UniProt (Q22XT1)	Tetrahymena thermophila SB210
3	C (C)	Myb-like DNA-binding domain protein	polymer	364	41937.1	1	UniProt (Q22VV9) Pfam (PF00249)	Tetrahymena thermophila SB210
4	D (D)	Transmembrane protein, putative	polymer	146	17043.3	1	UniProt (I7M8B9)	Tetrahymena thermophila SB210
5	E (E)	DNA (27-MER)	polymer	27	8426.5	1		synthetic construct
6	F (F)	DNA (27-MER)	polymer	27	8173.3	1		synthetic construct
7	G (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)

Sequence modifications

A: 1 - 372 (UniProt: Q22GC0)

PDB	External Database	Details
Gly -5	-	expression tag
Pro -4	-	expression tag
Glu -3	-	expression tag
Phe -2	-	expression tag
Lys -1	-	expression tag
Leu 0	-	expression tag

B: 1 - 324 (UniProt: Q22XT1)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
Pro 0	-	expression tag
Trp 325	-	expression tag
Ser 326	-	expression tag
His 327	-	expression tag
Pro 328	-	expression tag
Gln 329	-	expression tag
Phe 330	-	expression tag
Glu 331	-	expression tag
Lys 332	-	expression tag
Gly 333	-	expression tag
Gly 334	-	expression tag
Gly 335	-	expression tag
Ser 336	-	expression tag
Gly 337	-	expression tag
Gly 338	-	expression tag
Gly 339	-	expression tag
Ser 340	-	expression tag
Gly 341	-	expression tag
Gly 342	-	expression tag
Gly 343	-	expression tag
Ser 344	-	expression tag
Trp 345	-	expression tag
Ser 346	-	expression tag
His 347	-	expression tag
Pro 348	-	expression tag
Gln 349	-	expression tag
Phe 350	-	expression tag
Glu 351	-	expression tag
Lys 352	-	expression tag

C: 2 - 360 (UniProt: Q22VV9)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Pro 1	-	expression tag

D: 1 - 142 (UniProt: I7M8B9)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	160219.1
Non-Polymers*	Number of molecules	1
	Total formula weight	398.4
All*	Total formula weight	160617.5

*Water molecules are not included.