9U9E

Cryo-EM structure of hemimethylated DNA-bound Tetrahymena DNA methyltransferase complex MTA1c

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	mRNA m(6)A methyltransferase	polymer	378	43368.9	1	UniProt (Q22GC0) Pfam (PF05063)	Tetrahymena thermophila SB210
2	B (B)	MT-a70 family protein MTA9	polymer	485	55576.1	1	UniProt (I7MIF9)	Tetrahymena thermophila SB210
3	C (C)	Myb-like DNA-binding domain protein	polymer	364	41937.1	1	UniProt (Q22VV9) Pfam (PF00249)	Tetrahymena thermophila SB210
4	D (D)	Transmembrane protein, putative	polymer	146	17043.3	1	UniProt (I7M8B9)	Tetrahymena thermophila SB210
5	E (E)	DNA (27-MER)	polymer	27	8426.5	1		synthetic construct
6	F (F)	DNA (27-MER)	polymer	27	8173.3	1		synthetic construct
7	G (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)

Sequence modifications

A: 1 - 372 (UniProt: Q22GC0)

PDB	External Database	Details
Gly -5	-	expression tag
Pro -4	-	expression tag
Glu -3	-	expression tag
Phe -2	-	expression tag
Lys -1	-	expression tag
Leu 0	-	expression tag

B: 1 - 432 (UniProt: I7MIF9)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
Pro 0	-	expression tag
Phe 433	-	expression tag
Ala 434	-	expression tag
Ser 435	-	expression tag
Thr 436	-	expression tag
Asp 437	-	expression tag
Asn 438	-	expression tag
Tyr 439	-	expression tag
Arg 440	-	expression tag
Pro 441	-	expression tag
Gly 442	-	expression tag
Trp 443	-	expression tag
Ile 444	-	expression tag
Ser 445	-	expression tag
Val 446	-	expression tag
Ile 447	-	expression tag
Glu 448	-	expression tag
Lys 449	-	expression tag
Gly 450	-	expression tag
Ala 451	-	expression tag
Pro 452	-	expression tag
Trp 453	-	expression tag
Ser 454	-	expression tag
His 455	-	expression tag
Pro 456	-	expression tag
Gln 457	-	expression tag
Phe 458	-	expression tag
Glu 459	-	expression tag
Lys 460	-	expression tag
Gly 461	-	expression tag
Gly 462	-	expression tag
Gly 463	-	expression tag
Ser 464	-	expression tag
Gly 465	-	expression tag
Gly 466	-	expression tag
Gly 467	-	expression tag
Ser 468	-	expression tag
Gly 469	-	expression tag
Gly 470	-	expression tag
Gly 471	-	expression tag
Ser 472	-	expression tag
Trp 473	-	expression tag
Ser 474	-	expression tag
His 475	-	expression tag
Pro 476	-	expression tag
Gln 477	-	expression tag
Phe 478	-	expression tag
Glu 479	-	expression tag
Lys 480	-	expression tag

C: 2 - 360 (UniProt: Q22VV9)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Pro 1	-	expression tag

D: 1 - 142 (UniProt: I7M8B9)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	174525.3
Non-Polymers*	Number of molecules	1
	Total formula weight	398.4
All*	Total formula weight	174923.7

*Water molecules are not included.