9U9D
Bipartite Genetically Encoded Biosensor sG-GECO1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Myosin light chain kinase, smooth muscle, deglutamylated form,Green fluorescent protein,Calmodulin-1 | polymer | 234 | 26684.7 | 1 | UniProt (P11799) UniProt (P42212) UniProt (P0DP29) Pfam (PF13499) | synthetic construct | |
| 2 | B (B) | Green fluorescent protein | polymer | 234 | 26165.5 | 1 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria | |
| 3 | C, D (A, B) | water | water | 18.0 | 174 | Chemie (HOH) |
Sequence modifications
A: 1 - 36 (PDB: 9U9D)
A: 37 - 60 (UniProt: P11799)
A: 61 - 84 (UniProt: P42212)
A: 88 - 234 (UniProt: P0DP29)
B: 13 - 81 (UniProt: P42212)
B: 90 - 234 (UniProt: P42212)
A: 37 - 60 (UniProt: P11799)
| PDB | External Database | Details |
|---|---|---|
| Met 37 | Lys 1726 | conflict |
| Val 38 | Lys 1727 | conflict |
| Asp 39 | Tyr 1728 | conflict |
| Ser 40 | Met 1729 | conflict |
| Ser 41 | Ala 1730 | conflict |
| Tyr 46 | Gln 1735 | conflict |
| Val 54 | Ala 1743 | conflict |
| Leu 55 | Ile 1744 | conflict |
| Arg 56 | Gly 1745 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 70 | Lys 158 | conflict |
| Ala 75 | Val 163 | conflict |
| Gln 78 | Lys 166 | conflict |
| Cys 80 | Arg 168 | conflict |
| Leu 83 | Ile 171 | conflict |
| Val 84 | Glu 172 | conflict |
| Ser 85 | - | linker |
| Leu 86 | - | linker |
| Arg 87 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ile 98 | Phe 13 | conflict |
| Leu 101 | Ala 16 | conflict |
| Arg 116 | Lys 31 | conflict |
| Asp 146 | Asn 61 | conflict |
| Ala 156 | Thr 71 | conflict |
| Tyr 164 | Asp 79 | conflict |
| Arg 165 | Thr 80 | conflict |
| Thr 167 | Ser 82 | conflict |
| Tyr 175 | Phe 90 | conflict |
| Gly 176 | Arg 91 | conflict |
| Leu 211 | Ile 126 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ala 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Trp 4 | - | expression tag |
| Ser 5 | - | expression tag |
| His 6 | - | expression tag |
| Pro 7 | - | expression tag |
| Gln 8 | - | expression tag |
| Phe 9 | - | expression tag |
| Glu 10 | - | expression tag |
| Lys 11 | - | expression tag |
| Val 12 | - | expression tag |
| Ser 14 | Ile 171 | conflict |
| Met 15 | Glu 172 | conflict |
| Gly 18 | Ser 175 | conflict |
| Tyr 23 | Asp 180 | conflict |
| Thr 48 | Ser 205 | conflict |
| Lys 49 | Ala 206 | conflict |
| Arg 74 | His 231 | conflict |
| Pro 79 | Leu 236 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 82 | - | linker |
| Gly 83 | - | linker |
| Thr 84 | - | linker |
| Gly 85 | - | linker |
| Gly 86 | - | linker |
| Ser 87 | - | linker |
| Met 88 | - | linker |
| Val 89 | - | linker |
| Leu 152 | Phe 64 | conflict |
| Cro 154 | Ser 65 | chromophore |
| Cro 154 | Tyr 66 | chromophore |
| Cro 154 | Gly 67 | chromophore |
| Ile 181 | Val 93 | conflict |
| Phe 233 | Tyr 145 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 52850.2 | |
| All* | Total formula weight | 52850.2 |
