9U5E

cryo-EM structure of l-SPD bound D1R-Gs complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas	polymer	245	28790.5	1	UniProt (P63092) UniProt (Q5JWF2) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein,Extra large alphas protein,XLalphas
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody35	polymer	139	15271.9	1		Lama glama
5	E (R)	D(1A) dopamine receptor	polymer	446	49339.2	1	UniProt (P21728) Pfam (PF00001)	Homo sapiens (human)	Dopamine D1 receptor
6	F (R)	(13~{a}~{S})-3,9-dimethoxy-6,8,13,13~{a}-tetrahydro-5~{H}-isoquinolino[2,1-b]isoquinoline-2,10-diol	non-polymer		327.4	1	Chemie (A1EN9)

Sequence modifications

A: 9 - 60 (UniProt: P63092)

PDB	External Database	Details
Gly 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Gly 5	-	expression tag
Gly 6	-	expression tag
Ser 7	-	expression tag
Met 8	-	expression tag
Met 25	Lys 25	conflict
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict

A: 67 - 116 (UniProt: P63092)

PDB	External Database	Details
Arg 61	-	linker
Ile 62	-	linker
Tyr 63	-	linker
His 64	-	linker
Val 65	-	linker
Asn 66	-	linker
Ala 88	Gly 226	conflict
Asp 111	Ala 249	conflict
Asp 114	Ser 252	conflict

A: 117 - 246 (UniProt: Q5JWF2)

PDB	External Database	Details
Asp 124	Leu 915	conflict
Ser 218	Ala 1009	conflict
Ala 224	Ile 1015	conflict
Ile 227	Val 1018	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	139178.3
Non-Polymers*	Number of molecules	1
	Total formula weight	327.4
All*	Total formula weight	139505.6

*Water molecules are not included.