9U53

Crystal structure of the CYP154C2 Q230A from Streptomyces avermitilis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cytochrome P450	polymer	420	45215.4	1	UniProt (A0A4D4LTT1)	Streptomyces avermitilis
2	B (A)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
3	C, E (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4	D, F (A)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	G (A)	water	water		18.0	387	Chemie (HOH)

Sequence modifications

A: 1 - 400 (UniProt: A0A4D4LTT1)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 230	Gln 230	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45215.4
Non-Polymers*	Number of molecules	5
	Total formula weight	952.9
All*	Total formula weight	46168.2

*Water molecules are not included.