9U4W

cryo-EM structure of pig GnRHR bound with mammal GnRH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(q) subunit alpha-1	polymer	246	28084.8	1		Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	344	37784.3	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (F)	Gonadoliberin-1	polymer	11	1183.3	1	UniProt (P01148) Pfam (PF00446)	Homo sapiens	Gonadoliberin I,Gonadorelin,Gonadotropin-releasing hormone I,GnRH-I,Luliberin I,Luteinizing hormone-releasing hormone I,LH-RH I
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (R)	Beta-2 adrenergic receptor,Gonadotropin-releasing hormone receptor	polymer	414	47241.8	1	UniProt (P49922) Pfam (PF00001)	Homo sapiens (human)	GnRH receptor,GnRH-R,Luteinizing hormone-releasing hormone receptor,LHRH
6	F (S)	scFv16	polymer	285	30363.0	1		Homo sapiens (human)

Sequence modifications

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag

F: 1 - 10 (UniProt: P01148)

PDB	External Database	Details
Nh2 11	-	amidation

R: -85 - 0 (PDB: 9U4W)

PDB	External Database	Details
Met 1	-	linker
Ala 2	-	linker
Asn 3	-	linker
Ser 4	-	linker
Ala 5	-	linker
Ser 6	-	linker
Pro 7	-	linker
Glu 8	-	linker

R: 9 - 328 (UniProt: P49922)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	152518.4
All*	Total formula weight	152518.4

*Water molecules are not included.