9U40

Cryo-EM structure of peptide GPCR-Gq complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	exo-alpha-sialidase,Vasopressin V1a receptor	polymer	855	95944.0	1	UniProt (Q59310) UniProt (P37288) Pfam (PF00001)	Clostridium perfringens	V1aR,AVPR V1a,Antidiuretic hormone receptor 1a,Vascular/hepatic-type arginine vasopressin receptor
2	B (P)	Arg-vasopressin	polymer	10	1086.2	1	UniProt (P01185) Pfam (PF00220)	Homo sapiens	Arginine-vasopressin
3	C (A)	Guanine nucleotide-binding protein G(q) subunit alpha-1	polymer	246	28084.8	1		Homo sapiens (human)
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (N)	Nanobody 35	polymer	157	17057.3	1		Lama glama
7	G (R)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

R: -431 - 20 (UniProt: Q59310)

PDB	External Database	Details
Met -458	-	initiating methionine
Lys -457	-	expression tag
Thr -456	-	expression tag
Ile -455	-	expression tag
Ile -454	-	expression tag
Ala -453	-	expression tag
Leu -452	-	expression tag
Ser -451	-	expression tag
Tyr -450	-	expression tag
Ile -449	-	expression tag
Phe -448	-	expression tag
Cys -447	-	expression tag
Leu -446	-	expression tag
Val -445	-	expression tag
Phe -444	-	expression tag
Ala -443	-	expression tag
Asp -442	-	expression tag
Tyr -441	-	expression tag
Lys -440	-	expression tag
Asp -439	-	expression tag
Asp -438	-	expression tag
Asp -437	-	expression tag
Asp -436	-	expression tag
Ala -435	-	expression tag
Gly -434	-	expression tag
Arg -433	-	expression tag
Ala -432	-	expression tag
Ser -281	Gly 393	conflict

R: 21 - 378 (UniProt: P37288)

PDB	External Database	Details
Leu 379	-	expression tag
Glu 380	-	expression tag
Val 381	-	expression tag
Leu 382	-	expression tag
Phe 383	-	expression tag
Gln 384	-	expression tag
Gly 385	-	expression tag
Pro 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag
His 389	-	expression tag
His 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag

P: 1 - 9 (UniProt: P01185)

PDB	External Database	Details
Nh2 10	-	amidation

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Ser 359	-	expression tag
Gly 360	-	expression tag
Val 361	-	expression tag
Ser 362	-	expression tag
Gly 363	-	expression tag
Trp 364	-	expression tag
Arg 365	-	expression tag
Leu 366	-	expression tag
Phe 367	-	expression tag
Lys 368	-	expression tag
Lys 369	-	expression tag
Ile 370	-	expression tag
Ser 371	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	191089.3
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	191113.6

*Water molecules are not included.