9TWR

Complex of E. coli leucyl-tRNA synthetase AN3365-resistant mutant R344H bound to tRNA(leu) and AN3365

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Leucine--tRNA ligase	polymer	880	99497.0	1	UniProt (P07813) Pfam (PF09334) Pfam (PF13603) Pfam (PF00133) Pfam (PF08264)	Escherichia coli	Leucyl-tRNA synthetase,LeuRS
2	B (B)	tRNA(leu)	polymer	86	27723.4	1	GenBank (1845258627)	Escherichia coli
3	C (A)	(2~{S})-2-azanyl-~{N}-[azanyl-(3-fluorophenyl)-oxidanylidene-$l^{6}-sulfanylidene]-4-methyl-pentanamide	non-polymer		287.4	1	Chemie (A1JX6)
4	D (B)	[(1S,5R,6R,7'S,8R)-7'-(aminomethyl)-6-(6-aminopurin-9-yl)-2'-(3-oxidanylpropoxy)spiro[2,4,7-trioxa-3-boranuidabicyclo[3.3.0]octane-3,9'-8-oxa-9-boranuidabicyclo[4.3.0]nona-1(6),2,4-triene]-8-yl]methyl dihydrogen phosphate	non-polymer		565.3	1	Chemie (365) PubChem (137348125)
5	E, F (A, B)	water	water		18.0	518	Chemie (HOH)

Sequence modifications

A: 1 - 860 (UniProt: P07813)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 344	Arg 344	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	127220.4
Non-Polymers*	Number of molecules	2
	Total formula weight	852.6
All*	Total formula weight	128073.0

*Water molecules are not included.