9TW3
Cryo-EM structure of human VPS34-CI in complex with GABARAP - alternative conformation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Phosphatidylinositol 3-kinase catalytic subunit type 3 | polymer | 887 | 101680.3 | 1 | UniProt (Q8NEB9) | Homo sapiens (human) | PtdIns-3-kinase type 3,Phosphatidylinositol 3-kinase p100 subunit,Phosphoinositide-3-kinase class 3,hVps34 |
| 2 | B (B) | Phosphoinositide 3-kinase regulatory subunit 4 | polymer | 1371 | 154790.4 | 1 | UniProt (Q99570) | Homo sapiens (human) | PI3-kinase regulatory subunit 4,PI3-kinase p150 subunit,Phosphoinositide 3-kinase adaptor protein |
| 3 | C, G (C, G) | Beclin-1 | polymer | 450 | 51953.1 | 2 | UniProt (Q14457) | Homo sapiens (human) | Coiled-coil myosin-like BCL2-interacting protein,Protein GT197 |
| 4 | D (D) | Beclin 1-associated autophagy-related key regulator | polymer | 493 | 55461.3 | 1 | UniProt (Q6ZNE5) | Homo sapiens (human) | Barkor,Autophagy-related protein 14-like protein,Atg14L |
| 5 | E (E) | Nuclear receptor-binding factor 2 | polymer | 84 | 9716.2 | 1 | UniProt (Q96F24) | Homo sapiens (human) | NRBF-2,Comodulator of PPAR and RXR |
| 6 | F (F) | Gamma-aminobutyric acid receptor-associated protein | polymer | 119 | 14111.2 | 1 | UniProt (O95166) | Homo sapiens (human) | GABA(A) receptor-associated protein,MM46 |
| 7 | H (B) | MYRISTIC ACID | non-polymer | 228.4 | 1 | Chemie (MYR) PubChem (11005) | |||
| 8 | I (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 9 | J (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 10 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) PubChem (32051) |
Sequence modifications
B: 1 - 1358 (UniProt: Q99570)
D: 0 - 492 (UniProt: Q6ZNE5)
E: 1 - 79 (UniProt: Q96F24)
F: 1 - 116 (UniProt: O95166)
| PDB | External Database | Details |
|---|---|---|
| Ser 1359 | - | expression tag |
| Arg 1360 | - | expression tag |
| Pro 1361 | - | expression tag |
| Thr 1362 | - | expression tag |
| Thr 1363 | - | expression tag |
| Ala 1364 | - | expression tag |
| Ser 1365 | - | expression tag |
| Glu 1366 | - | expression tag |
| Asn 1367 | - | expression tag |
| Leu 1368 | - | expression tag |
| Tyr 1369 | - | expression tag |
| Phe 1370 | - | expression tag |
| Gln 1371 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Thr 1 | - | insertion |
| Ser 256 | Asn 256 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser -4 | - | expression tag |
| His -3 | - | expression tag |
| Met -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Val 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 439665.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 826.7 | |
| All* | Total formula weight | 440492.3 |
