9TL6

Cryo-EM Structure of the LPOR:Chlide:NADPH Complexes (RD-21) - improved resolution of a dimer building block form RF-21

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Protochlorophyllide reductase B, chloroplastic	polymer	353	38236.3	2	UniProt (P21218) Pfam (PF00106)	Arabidopsis thaliana (thale cress)	PCR B,NADPH-protochlorophyllide oxidoreductase B,POR B
2	C, F (A, B)	NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		745.4	2	Chemie (NDP)
3	D, H (A, B)	Chlorophyllide a	non-polymer		615.0	2	Chemie (A1JWG)
4	E, G (A, B)	1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE	non-polymer		787.2	2	Chemie (LMG) PubChem (446441)
5	I, J (A, B)	water	water		18.0	25	Chemie (HOH)

Sequence modifications

A, B: 68 - 401 (UniProt: P21218)

PDB	External Database	Details
Met 49	-	initiating methionine
Gly 50	-	expression tag
Ser 51	-	expression tag
Ser 52	-	expression tag
His 53	-	expression tag
His 54	-	expression tag
His 55	-	expression tag
His 56	-	expression tag
His 57	-	expression tag
His 58	-	expression tag
Ser 59	-	expression tag
Ser 60	-	expression tag
Gly 61	-	expression tag
Leu 62	-	expression tag
Val 63	-	expression tag
Pro 64	-	expression tag
Arg 65	-	expression tag
Gly 66	-	expression tag
Ser 67	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76472.6
Non-Polymers*	Number of molecules	6
	Total formula weight	4295.1
All*	Total formula weight	80767.8

*Water molecules are not included.