9TJW

Ternary complex of E. coli leucyl-tRNA synthetase mutant R178A, tRNA(leu) and the leucyl adenylate analog LeuAMS in the aminoacyl transfer state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Leucine--tRNA ligase	polymer	880	99429.9	2	UniProt (P07813)	Escherichia coli	Leucyl-tRNA synthetase,LeuRS
2	B, D (B, D)	tRNA(leu)	polymer	87	28052.7	2	GenBank (1231762938)	Escherichia coli
3	E, G (A, C)	5'-O-(L-leucylsulfamoyl)adenosine	non-polymer		459.5	2	Chemie (LSS) PubChem (5288690)
4	F (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
5	H, I, J, K (A, B, C, D)	water	water		18.0	690	Chemie (HOH)

Sequence modifications

A, C: 1 - 860 (UniProt: P07813)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 178	Arg 178	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	254965.1
Non-Polymers*	Number of molecules	3
	Total formula weight	943.3
All*	Total formula weight	255908.4

*Water molecules are not included.