9TIA

G14 variant of 57aBi scaffold crystallized in P6422

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	PIH1 domain-containing protein 1	polymer	150	16956.3	1	UniProt (Q9NWS0) Pfam (PF08190)	Homo sapiens (human)	Nucleolar protein 17 homolog, G14 variant of 57aBi scaffold based on PIH1D1
2	B (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
3	C (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA) PubChem (271)
4	D (A)	water	water		18.0	40	Chemie (HOH)

Sequence modifications

A: 21 - 150 (UniProt: Q9NWS0)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Ser 9	-	expression tag
Ala 10	-	expression tag
Ala 11	-	expression tag
Leu 12	-	expression tag
Glu 13	-	expression tag
Val 14	-	expression tag
Leu 15	-	expression tag
Phe 16	-	expression tag
Gln 17	-	expression tag
Gly 18	-	expression tag
Pro 19	-	expression tag
Gly 20	-	expression tag
Ser 110	Glu 140	engineered mutation
Arg 114	Thr 144	engineered mutation
Leu 117	Arg 147	engineered mutation
Ser 118	Glu 148	engineered mutation
Trp 122	Asp 152	engineered mutation
Ser 125	Asn 155	engineered mutation
Trp 127	Gln 157	engineered mutation
Phe 129	Asn 159	engineered mutation
Ala 131	Glu 161	engineered mutation
Val 133	Arg 163	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	16956.3
Non-Polymers*	Number of molecules	2
	Total formula weight	190.3
All*	Total formula weight	17146.6

*Water molecules are not included.