9TDV

Structure of D81A-Fructofuranosidase from Purpureocilum lilacinum in complex with raffinose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Invertase	polymer	576	62664.2	4	UniProt (A0A179H0Q0) Pfam (PF00251) Pfam (PF08244)	Komagataella phaffii CBS 7435
2	E, I (E, I)	alpha-D-galactopyranose-(1-6)-alpha-D-glucopyranose	branched		342.3	2	In PDB GlyTouCan (G54976AD)
3	F, G, H, J (F, G, H, J)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	4	In PDB GlyTouCan (G83582BK)
4	IA, K, Q, Y (D, A, B, C)	beta-D-fructofuranose	non-polymer		180.2	4	Chemie (FRU)
5	BA, JA, L (C, D, A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	3	Chemie (TRS)
6	EA, FA, GA, HA, M... (C, A, D, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	16	Chemie (NAG)
7	R, Z (B, C)	alpha-D-glucopyranose	non-polymer		180.2	2	Chemie (GLC)
8	AA, S (C, B)	alpha-D-galactopyranose	non-polymer		180.2	2	Chemie (GLA)
9	CA, DA, KA, LA, T (C, D, B)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
10	QA, RA, SA, TA (A, B, C, D)	water	water		18.0	1104	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 576 (UniProt: A0A179H0Q0)

PDB	External Database	Details
Ala 81	Asp 81	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	250656.6
Branched	Number of molecules	6
	Total formula weight	6273.6
Non-Polymers*	Number of molecules	32
	Total formula weight	5827.3
All*	Total formula weight	262757.5

*Water molecules are not included.