9TAW

Structure of the human inner kinetochore CCAN bound to DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (82-MER)	polymer	171	52725.8	1		Homo sapiens
2	B (B)	DNA (80-MER)	polymer	171	52822.8	1		Homo sapiens
3	C (H)	Centromere protein H	polymer	247	28520.9	1	UniProt (Q9H3R5)	Homo sapiens (human)
4	D (I)	Centromere protein I	polymer	762	87615.0	1	UniProt (Q92674)	Homo sapiens (human)	CENP-I,FSH primary response protein 1,Follicle-stimulating hormone primary response protein,Interphase centromere complex protein 19,Leucine-rich primary response protein 1
5	E (K)	Centromere protein K	polymer	269	31696.1	1	UniProt (Q9BS16)	Homo sapiens (human)
6	F (L)	Centromere protein L	polymer	348	39364.0	1	UniProt (Q8N0S6)	Homo sapiens (human)	CENP-L,Interphase centromere complex protein 33
7	G (M)	Centromere protein M	polymer	180	19761.9	1	UniProt (Q9NSP4)	Homo sapiens (human)
8	H (N)	Centromere protein N	polymer	339	39609.6	1	UniProt (Q96H22)	Homo sapiens (human)
9	I (O)	Centromere protein O	polymer	300	33830.6	1	UniProt (Q9BU64)	Homo sapiens (human)	CENP-O,Interphase centromere complex protein 36
10	J (P)	Centromere protein P	polymer	288	33210.9	1	UniProt (Q6IPU0)	Homo sapiens (human)
11	K (Q)	Centromere protein Q	polymer	215	24634.5	1	UniProt (Q7L2Z9)	Homo sapiens (human)	CENP-Q
12	L (R)	Centromere protein R	polymer	177	20228.3	1	UniProt (Q13352)	Homo sapiens (human)
13	M (S)	Centromere protein S	polymer	138	15917.9	1	UniProt (Q8N2Z9)	Homo sapiens (human)
14	N (T)	Centromere protein T	polymer	777	84067.7	1	UniProt (Q96BT3)	Homo sapiens (human)	CENP-T,Interphase centromere complex protein 22
15	O (U)	Centromere protein U	polymer	211	24484.9	1	UniProt (Q71F23)	Homo sapiens (human)	CENP-U,Centromere protein of 50 kDa,CENP-50,Interphase centromere complex protein 24,KSHV latent nuclear antigen-interacting protein 1,MLF1-interacting protein,Polo-box-interacting protein 1
16	P (W)	Centromere protein W	polymer	88	10087.2	1	UniProt (Q5EE01)	Homo sapiens (human)
17	Q (X)	Centromere protein X	polymer	81	8972.4	1	UniProt (A8MT69)	Homo sapiens (human)
18	R (b)	Centromere protein C,Centromere protein C,Centromere protein C,Centromere protein C,Methylated-DNA--protein-cysteine methyltransferase	polymer	775	86473.1	1	UniProt (Q03188)	Homo sapiens (human)

Sequence modifications

I: 1 - 756 (UniProt: Q92674)

PDB	External Database	Details
Glu 757	-	expression tag
Asn 758	-	expression tag
Leu 759	-	expression tag
Tyr 760	-	expression tag
Phe 761	-	expression tag
Gln 762	-	expression tag

L: 1 - 344 (UniProt: Q8N0S6)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Leu -1	-	expression tag
Gly 0	-	expression tag

O: 89 - 300 (UniProt: Q9BU64)

PDB	External Database	Details
Met 1	-	initiating methionine
Glu 2	-	expression tag
Gln 3	-	expression tag
Ala 4	-	expression tag
Asn 5	-	expression tag
Pro 6	-	expression tag
Leu 7	-	expression tag
Arg 8	-	expression tag
Pro 9	-	expression tag
Asp 10	-	expression tag
Gly 11	-	expression tag
Glu 12	-	expression tag
Ser 13	-	expression tag
Lys 14	-	expression tag
Gly 15	-	expression tag
Gly 16	-	expression tag
Val 17	-	expression tag
Leu 18	-	expression tag
Ala 19	-	expression tag
His 20	-	expression tag
Leu 21	-	expression tag
Glu 22	-	expression tag
Arg 23	-	expression tag
Leu 24	-	expression tag
Glu 25	-	expression tag
Thr 26	-	expression tag
Gln 27	-	expression tag
Val 28	-	expression tag
Ser 29	-	expression tag
Arg 30	-	expression tag
Ser 31	-	expression tag
Arg 32	-	expression tag
Lys 33	-	expression tag
Gln 34	-	expression tag
Ser 35	-	expression tag
Glu 36	-	expression tag
Glu 37	-	expression tag
Leu 38	-	expression tag
Gln 39	-	expression tag
Ser 40	-	expression tag
Val 41	-	expression tag
Gln 42	-	expression tag
Ala 43	-	expression tag
Gln 44	-	expression tag
Glu 45	-	expression tag
Gly 46	-	expression tag
Ala 47	-	expression tag
Leu 48	-	expression tag
Gly 49	-	expression tag
Thr 50	-	expression tag
Lys 51	-	expression tag
Ile 52	-	expression tag
His 53	-	expression tag
Lys 54	-	expression tag
Leu 55	-	expression tag
Arg 56	-	expression tag
Arg 57	-	expression tag
Leu 58	-	expression tag
Arg 59	-	expression tag
Asp 60	-	expression tag
Glu 61	-	expression tag
Leu 62	-	expression tag
Arg 63	-	expression tag
Ala 64	-	expression tag
Val 65	-	expression tag
Val 66	-	expression tag
Arg 67	-	expression tag
His 68	-	expression tag
Arg 69	-	expression tag
Arg 70	-	expression tag
Ala 71	-	expression tag
Ser 72	-	expression tag
Val 73	-	expression tag
Lys 74	-	expression tag
Ala 75	-	expression tag
Cys 76	-	expression tag
Ile 77	-	expression tag
Ala 78	-	expression tag
Asn 79	-	expression tag
Val 80	-	expression tag
Glu 81	-	expression tag
Pro 82	-	expression tag
Asn 83	-	expression tag
Gln 84	-	expression tag
Thr 85	-	expression tag
Val 86	-	expression tag
Glu 87	-	expression tag
Ile 88	-	expression tag

Q: 55 - 268 (UniProt: Q7L2Z9)

PDB	External Database	Details
Met 54	-	initiating methionine

T: 1 - 561 (UniProt: Q96BT3)

PDB	External Database	Details
Met -215	-	initiating methionine
Ser -214	-	expression tag
Tyr -213	-	expression tag
Tyr -212	-	expression tag
His -211	-	expression tag
His -210	-	expression tag
His -209	-	expression tag
His -208	-	expression tag
His -207	-	expression tag
His -206	-	expression tag
Asp -205	-	expression tag
Tyr -204	-	expression tag
Asp -203	-	expression tag
Ile -202	-	expression tag
Pro -201	-	expression tag
Thr -200	-	expression tag
Thr -199	-	expression tag
Glu -198	-	expression tag
Asn -197	-	expression tag
Leu -196	-	expression tag
Tyr -195	-	expression tag
Phe -194	-	expression tag
Gln -193	-	expression tag
Gly -192	-	expression tag
Met -191	-	expression tag
Asp -190	-	expression tag
Lys -189	-	expression tag
Asp -188	-	expression tag
Cys -187	-	expression tag
Glu -186	-	expression tag
Met -185	-	expression tag
Lys -184	-	expression tag
Arg -183	-	expression tag
Thr -182	-	expression tag
Thr -181	-	expression tag
Leu -180	-	expression tag
Asp -179	-	expression tag
Ser -178	-	expression tag
Pro -177	-	expression tag
Leu -176	-	expression tag
Gly -175	-	expression tag
Lys -174	-	expression tag
Leu -173	-	expression tag
Glu -172	-	expression tag
Leu -171	-	expression tag
Ser -170	-	expression tag
Gly -169	-	expression tag
Cys -168	-	expression tag
Glu -167	-	expression tag
Gln -166	-	expression tag
Gly -165	-	expression tag
Leu -164	-	expression tag
His -163	-	expression tag
Arg -162	-	expression tag
Ile -161	-	expression tag
Ile -160	-	expression tag
Phe -159	-	expression tag
Leu -158	-	expression tag
Gly -157	-	expression tag
Lys -156	-	expression tag
Gly -155	-	expression tag
Thr -154	-	expression tag
Ser -153	-	expression tag
Ala -152	-	expression tag
Ala -151	-	expression tag
Asp -150	-	expression tag
Ala -149	-	expression tag
Val -148	-	expression tag
Glu -147	-	expression tag
Val -146	-	expression tag
Pro -145	-	expression tag
Ala -144	-	expression tag
Pro -143	-	expression tag
Ala -142	-	expression tag
Ala -141	-	expression tag
Val -140	-	expression tag
Leu -139	-	expression tag
Gly -138	-	expression tag
Gly -137	-	expression tag
Pro -136	-	expression tag
Glu -135	-	expression tag
Pro -134	-	expression tag
Leu -133	-	expression tag
Met -132	-	expression tag
Gln -131	-	expression tag
Ala -130	-	expression tag
Thr -129	-	expression tag
Ala -128	-	expression tag
Trp -127	-	expression tag
Leu -126	-	expression tag
Asn -125	-	expression tag
Ala -124	-	expression tag
Tyr -123	-	expression tag
Phe -122	-	expression tag
His -121	-	expression tag
Gln -120	-	expression tag
Pro -119	-	expression tag
Glu -118	-	expression tag
Ala -117	-	expression tag
Ile -116	-	expression tag
Glu -115	-	expression tag
Glu -114	-	expression tag
Phe -113	-	expression tag
Pro -112	-	expression tag
Val -111	-	expression tag
Pro -110	-	expression tag
Ala -109	-	expression tag
Leu -108	-	expression tag
His -107	-	expression tag
His -106	-	expression tag
Pro -105	-	expression tag
Val -104	-	expression tag
Phe -103	-	expression tag
Gln -102	-	expression tag
Gln -101	-	expression tag
Glu -100	-	expression tag
Ser -99	-	expression tag
Phe -98	-	expression tag
Thr -97	-	expression tag
Arg -96	-	expression tag
Gln -95	-	expression tag
Val -94	-	expression tag
Leu -93	-	expression tag
Trp -92	-	expression tag
Lys -91	-	expression tag
Leu -90	-	expression tag
Leu -89	-	expression tag
Lys -88	-	expression tag
Val -87	-	expression tag
Val -86	-	expression tag
Lys -85	-	expression tag
Phe -84	-	expression tag
Gly -83	-	expression tag
Glu -82	-	expression tag
Val -81	-	expression tag
Ile -80	-	expression tag
Ser -79	-	expression tag
Tyr -78	-	expression tag
Ser -77	-	expression tag
His -76	-	expression tag
Leu -75	-	expression tag
Ala -74	-	expression tag
Ala -73	-	expression tag
Leu -72	-	expression tag
Ala -71	-	expression tag
Gly -70	-	expression tag
Asn -69	-	expression tag
Pro -68	-	expression tag
Ala -67	-	expression tag
Ala -66	-	expression tag
Thr -65	-	expression tag
Ala -64	-	expression tag
Ala -63	-	expression tag
Val -62	-	expression tag
Lys -61	-	expression tag
Thr -60	-	expression tag
Ala -59	-	expression tag
Leu -58	-	expression tag
Ser -57	-	expression tag
Gly -56	-	expression tag
Asn -55	-	expression tag
Pro -54	-	expression tag
Val -53	-	expression tag
Pro -52	-	expression tag
Ile -51	-	expression tag
Leu -50	-	expression tag
Ile -49	-	expression tag
Pro -48	-	expression tag
Cys -47	-	expression tag
His -46	-	expression tag
Arg -45	-	expression tag
Val -44	-	expression tag
Val -43	-	expression tag
Gln -42	-	expression tag
Gly -41	-	expression tag
Asp -40	-	expression tag
Leu -39	-	expression tag
Asp -38	-	expression tag
Val -37	-	expression tag
Gly -36	-	expression tag
Gly -35	-	expression tag
Tyr -34	-	expression tag
Glu -33	-	expression tag
Gly -32	-	expression tag
Gly -31	-	expression tag
Leu -30	-	expression tag
Ala -29	-	expression tag
Val -28	-	expression tag
Lys -27	-	expression tag
Glu -26	-	expression tag
Trp -25	-	expression tag
Leu -24	-	expression tag
Leu -23	-	expression tag
Ala -22	-	expression tag
His -21	-	expression tag
Glu -20	-	expression tag
Gly -19	-	expression tag
His -18	-	expression tag
Arg -17	-	expression tag
Leu -16	-	expression tag
Gly -15	-	expression tag
Lys -14	-	expression tag
Pro -13	-	expression tag
Gly -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Asp -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

U: 243 - 418 (UniProt: Q71F23)

PDB	External Database	Details
Glu 419	-	expression tag
Asn 420	-	expression tag
Leu 421	-	expression tag
Tyr 422	-	expression tag
Phe 423	-	expression tag
Gln 424	-	expression tag
Ser 425	-	expression tag
Trp 426	-	expression tag
Ser 427	-	expression tag
His 428	-	expression tag
Pro 429	-	expression tag
Gln 430	-	expression tag
Phe 431	-	expression tag
Glu 432	-	expression tag
Lys 433	-	expression tag
Gly 434	-	expression tag
Gly 435	-	expression tag
Gly 436	-	expression tag
Ser 437	-	expression tag
Gly 438	-	expression tag
Gly 439	-	expression tag
Gly 440	-	expression tag
Ser 441	-	expression tag
Gly 442	-	expression tag
Gly 443	-	expression tag
Gly 444	-	expression tag
Ser 445	-	expression tag
Trp 446	-	expression tag
Ser 447	-	expression tag
His 448	-	expression tag
Pro 449	-	expression tag
Gln 450	-	expression tag
Phe 451	-	expression tag
Glu 452	-	expression tag
Lys 453	-	expression tag

b: 1 - 545 (UniProt: Q03188)
b: 546 - 775 (PDB: 9TAW)

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	694023.8
All*	Total formula weight	694023.8

*Water molecules are not included.