9TAM
E. coli Complex I D79N NuoA mutant purified in LMNG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | NADH-quinone oxidoreductase subunit C/D | polymer | 600 | 68780.5 | 1 | UniProt (P33599) Pfam (PF00329) Pfam (PF00346) | Escherichia coli BL21(DE3) | NADH dehydrogenase I subunit C/D,NDH-1 subunit C/D,NUO3/NUO4 |
| 2 | B (E) | NADH-quinone oxidoreductase subunit E | polymer | 166 | 18614.0 | 1 | UniProt (P0AFD1) Pfam (PF01257) | Escherichia coli BW25113 | NADH dehydrogenase I subunit E,NDH-1 subunit E,NUO5 |
| 3 | C (F) | NADH-quinone oxidoreductase subunit F | polymer | 461 | 51208.4 | 1 | UniProt (P31979) Pfam (PF01512) Pfam (PF10589) | Escherichia coli BW25113 | NADH dehydrogenase I subunit F,NDH-1 subunit F,NUO6 |
| 4 | D (G) | NADH-quinone oxidoreductase subunit G | polymer | 910 | 100663.6 | 1 | UniProt (P33602) Pfam (PF22117) Pfam (PF04879) Pfam (PF00384) Pfam (PF01568) Pfam (PF13510) Pfam (PF10588) | Escherichia coli BW25113 | NADH dehydrogenase I subunit G,NDH-1 subunit G,NUO7 |
| 5 | E (I) | NADH-quinone oxidoreductase subunit I | polymer | 180 | 20562.8 | 1 | UniProt (P0AFD6) Pfam (PF00037) | Escherichia coli BL21(DE3) | NADH dehydrogenase I subunit I,NDH-1 subunit I,NUO9 |
| 6 | F (J) | NADH-quinone oxidoreductase subunit J | polymer | 184 | 19889.6 | 1 | UniProt (P0AFE0) Pfam (PF00499) | Escherichia coli BW25113 | NADH dehydrogenase I subunit J,NDH-1 subunit J,NUO10 |
| 7 | G (K) | NADH-quinone oxidoreductase subunit K | polymer | 100 | 10853.0 | 1 | UniProt (P0AFE4) Pfam (PF00420) | Escherichia coli BW25113 | NADH dehydrogenase I subunit K,NDH-1 subunit K,NUO11 |
| 8 | H (L) | NADH-quinone oxidoreductase subunit L | polymer | 613 | 66483.6 | 1 | UniProt (P33607) Pfam (PF00662) Pfam (PF00361) | Escherichia coli BW25113 | NADH dehydrogenase I subunit L,NDH-1 subunit L,NUO12 |
| 9 | I (M) | NADH-quinone oxidoreductase subunit M | polymer | 509 | 56560.1 | 1 | UniProt (P0AFE8) Pfam (PF00361) | Escherichia coli BW25113 | NADH dehydrogenase I subunit M,NDH-1 subunit M,NUO13 |
| 10 | J (N) | NADH-quinone oxidoreductase subunit N | polymer | 485 | 52072.7 | 1 | UniProt (P0AFF0) Pfam (PF00361) | Escherichia coli BW25113 | NADH dehydrogenase I subunit N,NDH-1 subunit N,NUO14 |
| 11 | K (B) | NADH-quinone oxidoreductase subunit B | polymer | 220 | 25081.8 | 1 | UniProt (P0AFC7) Pfam (PF01058) | Escherichia coli BW25113 | NADH dehydrogenase I subunit B,NDH-1 subunit B,NUO2 |
| 12 | L (H) | NADH-quinone oxidoreductase subunit H | polymer | 325 | 36240.9 | 1 | UniProt (P0AFD4) Pfam (PF00146) | Escherichia coli BW25113 | NADH dehydrogenase I subunit H,NDH-1 subunit H,NUO8 |
| 13 | M (A) | NADH-quinone oxidoreductase subunit A | polymer | 147 | 16473.3 | 1 | UniProt (P0AFC3) Pfam (PF00507) | Escherichia coli BW25113 | NADH dehydrogenase I subunit A,NDH-1 subunit A,NUO1 |
| 14 | N, T (E, G) | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 15 | O, OA, Q, R, S... (F, B, G, I) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 7 | Chemie (SF4) | |||
| 16 | P (F) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 1 | Chemie (FMN) PubChem (710) PubChem (23585816) PubChem (8965) PubChem (51435500) PubChem (51435503) PubChem (643976) PubChem (5702760) PubChem (7048779) PubChem (7048781) PubChem (15559251) | |||
| 17 | U, V, Y (G, I) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) PubChem (271) | |||
| 18 | CA, DA, EA, FA, IA... (L, M, N, H, A...) | 1,2-Distearoyl-sn-glycerophosphoethanolamine | non-polymer | 748.1 | 12 | Chemie (3PE) | |||
| 19 | AA, BA, GA, HA, RA (J, M, H) | (1R)-2-(dodecanoyloxy)-1-[(phosphonooxy)methyl]ethyl tetradecanoate | non-polymer | 564.7 | 5 | Chemie (7PH) PubChem (24779689) PubChem (138276598) | |||
| 20 | MA, NA (N) | Ubiquinone-8 | non-polymer | 727.1 | 2 | Chemie (UQ8) PubChem (1157) PubChem (176478936) PubChem (5283546) | |||
| 21 | AB, BB, CB, DB, EB... (L, M, N, B, H...) | water | water | 18.0 | 416 | Chemie (HOH) |
Sequence modifications
C: 5 - 600 (UniProt: P33599)
F: 2 - 445 (UniProt: P31979)
G: 3 - 910 (UniProt: P33602)
A: 1 - 147 (UniProt: P0AFC3)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Val 2 | - | expression tag |
| Asn 3 | - | expression tag |
| Asn 4 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Arg -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Ser -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| Thr -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Arg 0 | - | expression tag |
| Ala 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Leu 2 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 79 | Asp 79 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 543484.1 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 16644.4 | |
| All* | Total formula weight | 560128.5 |
