9T92

cryo-EM structure of autophosphorylated mTOR complex 2, overall refinement

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Serine/threonine-protein kinase mTOR	polymer	2590	293557.7	2	UniProt (P42345) Pfam (PF11865) Pfam (PF02259) Pfam (PF23593) Pfam (PF08771) Pfam (PF00454) Pfam (PF02260)	Homo sapiens (human)	FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin,mTOR,Mechanistic target of rapamycin,Rapamycin and FKBP12 target 1,Rapamycin target protein 1,Tyrosine-protein kinase mTOR
2	C, D (C, D)	Target of rapamycin complex subunit LST8	polymer	326	35910.1	2	UniProt (Q9BVC4) Pfam (PF00400)	Homo sapiens (human)	TORC subunit LST8,G protein beta subunit-like,Protein GbetaL,Mammalian lethal with SEC13 protein 8,mLST8
3	E, F (E, F)	Rapamycin-insensitive companion of mTOR	polymer	1734	195552.0	2	UniProt (Q6R327) Pfam (PF14664) Pfam (PF14666) Pfam (PF14663) Pfam (PF14668) Pfam (PF14665)	Homo sapiens (human)	AVO3 homolog,hAVO3
4	G, H (G, H)	Target of rapamycin complex 2 subunit MAPKAP1	polymer	522	59181.6	2	UniProt (Q9BPZ7) Pfam (PF05422) Pfam (PF16978) Pfam (PF25322) Pfam (PF16979)	Homo sapiens (human)	TORC2 subunit MAPKAP1,Mitogen-activated protein kinase 2-associated protein 1,Stress-activated map kinase-interacting protein 1,SAPK-interacting protein 1,mSIN1
5	I, J (A, B)	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	2	Chemie (IHP)
6	K, L (E, F)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)

Sequence modifications

A, B: 1 - 2549 (UniProt: P42345)

PDB	External Database	Details
Met -40	-	initiating methionine
Ala -39	-	expression tag
Ser -38	-	expression tag
Trp -37	-	expression tag
Ser -36	-	expression tag
His -35	-	expression tag
Pro -34	-	expression tag
Gln -33	-	expression tag
Phe -32	-	expression tag
Glu -31	-	expression tag
Lys -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ala -26	-	expression tag
Arg -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Trp -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
Pro -14	-	expression tag
Gln -13	-	expression tag
Phe -12	-	expression tag
Glu -11	-	expression tag
Lys -10	-	expression tag
Gly -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

E, F: 1 - 1708 (UniProt: Q6R327)

PDB	External Database	Details
Met -25	-	initiating methionine
Ala -24	-	expression tag
Asp -23	-	expression tag
Tyr -22	-	expression tag
Lys -21	-	expression tag
Asp -20	-	expression tag
His -19	-	expression tag
Asp -18	-	expression tag
Gly -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
His -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

G, H: 2 - 522 (UniProt: Q9BPZ7)

PDB	External Database	Details
Ace 1	-	acetylation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	1168402.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1450.9
All*	Total formula weight	1169853.6

*Water molecules are not included.