9T7P

Cryo-EM structure of ESG-2-36-bound D3 dopamine receptor with mini-Go

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	D(3) dopamine receptor,Lgbit	polymer	594	65118.7	1	UniProt (P35462)	Homo sapiens (human)	Dopamine D3 receptor
2	B (A)	Engineered miniGo,Guanine nucleotide-binding protein G(o) subunit alpha,Guanine nucleotide-binding protein G(o) subunit alpha	polymer	226	25248.9	1	UniProt (P09471)	Homo sapiens
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	384	41729.6	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
5	E (S)	scFv16	polymer	256	27340.5	1		Mus musculus
6	F (R)	3-[4-[2-[4-(2-fluoranyl-3-methoxy-phenyl)piperazin-1-yl]ethyl]cyclohexyl]-1,1-dimethyl-urea	non-polymer		406.5	1	Chemie (A1JUA)

Sequence modifications

R: 1 - 400 (UniProt: P35462)

PDB	External Database	Details
Asp -20	-	expression tag
Tyr -19	-	expression tag
Lys -18	-	expression tag
Asp -17	-	expression tag
Asp -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Lys -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 401	-	linker
Ser 402	-	linker
Ser 403	-	linker
Gly 404	-	linker
Gly 405	-	linker
Gly 406	-	linker
Gly 407	-	linker
Ser 408	-	linker
Gly 409	-	linker
Gly 410	-	linker
Gly 411	-	linker
Gly 412	-	linker
Ser 413	-	linker
Ser 414	-	linker
Gly 415	-	linker

R: 416 - 573 (PDB: 9T7P)
A: 1 - 55 (PDB: 9T7P)

PDB	External Database	Details
Gly 56	-	linker
Gly 57	-	linker
Ser 58	-	linker
Gly 59	-	linker
Gly 60	-	linker
Ser 61	-	linker
Gly 62	-	linker
Gly 63	-	linker

A: 64 - 226 (UniProt: P09471)

PDB	External Database	Details
Asp 109	Ala 227	conflict
Asp 112	Gly 230	conflict
-	Asp 232	deletion
-	Gln 233	deletion
-	Val 234	deletion
-	Leu 235	deletion
-	His 236	deletion
-	Glu 237	deletion
-	Asp 238	deletion
-	Glu 239	deletion
-	Thr 240	deletion
-	Thr 241	deletion
Ala 204	Ile 332	conflict
Ile 207	Val 335	conflict

B: 1 - 339 (UniProt: P62873)

PDB	External Database	Details
Met -18	-	initiating methionine
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Val -9	-	expression tag
Leu -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Pro -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Gly 340	-	expression tag
Ser 341	-	expression tag
Ser 342	-	expression tag
Gly 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Gly 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Ser 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Val 355	-	expression tag
Ser 356	-	expression tag
Gly 357	-	expression tag
Trp 358	-	expression tag
Arg 359	-	expression tag
Leu 360	-	expression tag
Phe 361	-	expression tag
Lys 362	-	expression tag
Lys 363	-	expression tag
Ile 364	-	expression tag
Ser 365	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	167298.8
Non-Polymers*	Number of molecules	1
	Total formula weight	406.5
All*	Total formula weight	167705.4

*Water molecules are not included.