9T6N

Cryo-EM structure of the human GANP-PCID2-DSS1 complex bound to UAP56

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Spliceosome RNA helicase DDX39B	polymer	432	49412.6	1	UniProt (Q13838) Pfam (PF00270) Pfam (PF00271)	Homo sapiens (human)	56 kDa U2AF65-associated protein,ATP-dependent RNA helicase p47,DEAD box protein UAP56,HLA-B-associated transcript 1 protein
2	B (C)	Germinal-center associated nuclear protein	polymer	405	46363.9	1	UniProt (O60318) Pfam (PF03399)	Homo sapiens (human)	GANP,80 kDa MCM3-associated protein,MCM3 acetylating protein,MCM3AP,MCM3 acetyltransferase
3	C (D)	PCI domain-containing protein 2	polymer	403	46458.3	1	UniProt (Q5JVF3) Pfam (PF01399)	Homo sapiens (human)	CSN12-like protein
4	D (E)	26S proteasome complex subunit SEM1	polymer	70	8284.6	1	UniProt (P60896) Pfam (PF05160)	Homo sapiens (human)	26S proteasome complex subunit DSS1,Deleted in split hand/split foot protein 1,Split hand/foot deleted protein 1,Split hand/foot malformation type 1 protein
5	E (B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
6	F (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

B: 1 - 428 (UniProt: Q13838)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

C: 99 - 499 (UniProt: O60318)

PDB	External Database	Details
Gly 95	-	expression tag
Pro 96	-	expression tag
Asp 97	-	expression tag
Met 98	-	expression tag

D: 1 - 399 (UniProt: Q5JVF3)

PDB	External Database	Details
Met -3	-	initiating methionine
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	150519.4
Non-Polymers*	Number of molecules	2
	Total formula weight	451.5
All*	Total formula weight	150970.9

*Water molecules are not included.