9T5Z
Cryo-EM structure of alphaM/beta2:MEM148-Fab headpiece complex (without alphaM I-domain)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Integrin alpha-M | polymer | 762 | 83912.8 | 1 | UniProt (P11215) | Homo sapiens (human) | CD11 antigen-like family member B,CR-3 alpha chain,Cell surface glycoprotein MAC-1 subunit alpha,Leukocyte adhesion receptor MO1,Neutrophil adherence receptor |
| 2 | B (B) | Integrin beta-2 | polymer | 469 | 52217.1 | 1 | UniProt (P05107) | Homo sapiens (human) | Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta,Complement receptor C3 subunit beta |
| 3 | C (H) | Murine IgG1 MEM148 heavy chain | polymer | 218 | 23865.5 | 1 | Mus musculus | ||
| 4 | D (L) | Anti-colorectal carcinoma light chain | polymer | 214 | 23936.6 | 1 | Mus musculus | ||
| 5 | E (C) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 6 | F, G (D, E) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 2 | In PDB GlyTouCan (G22768VO) | |||
| 7 | H (F) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 8 | I, J, K, M, N (A, B) | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) | |||
| 9 | L (B) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 10 | O, P (B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 11 | Q, R (A, B) | water | water | 18.0 | 7 | Chemie (HOH) |
Sequence modifications
A: 1 - 755 (UniProt: P11215)
B: 1 - 463 (UniProt: P05107)
| PDB | External Database | Details |
|---|---|---|
| Arg 224 | Asn 240 | engineered mutation |
| Thr 485 | Ala 501 | engineered mutation |
| Arg 680 | Asn 696 | engineered mutation |
| Thr 754 | Cys 770 | engineered mutation |
| Gly 756 | - | expression tag |
| Leu 757 | - | expression tag |
| Glu 758 | - | expression tag |
| Val 759 | - | expression tag |
| Leu 760 | - | expression tag |
| Phe 761 | - | expression tag |
| Gln 762 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 461 | Cys 483 | engineered mutation |
| Ala 462 | Gln 484 | engineered mutation |
| Ala 463 | Thr 485 | engineered mutation |
| Leu 464 | - | expression tag |
| Gln 465 | - | expression tag |
| Thr 466 | - | expression tag |
| Leu 467 | - | expression tag |
| Phe 468 | - | expression tag |
| Gln 469 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 183932.1 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 2832.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 697.7 | |
| All* | Total formula weight | 187462.4 |
